{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE293nnn/GSE293919/"]},"type":"primary"},"statusCodeValue":200,"statusCode":"OK"}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Mus musculus"," Homo sapiens"],"gds_type":[" Other"," Non-coding RNA profiling by high throughput sequencing","Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE293919"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Longitudinal monitoring of cytoplasmic RBP-RNA interactions and transcriptome in living cells by engineered protein nanocages","description":"Most existing RNA sequencing methods rely on cell lysis or fixation, limiting their use in longitudinal studies of the same cell population. Here, we introduce POND-seq (Protein nanocage-empOwered Non-Destructive sequencing), a strategy that employs secretory protein nanocages fused to RNA-binding proteins (RBPs) to recover RBP-associated RNAs from living cells. POND-seq robustly identifies RNA targets of cytoplasmic RBPs across multiple cell types and enables longitudinal tracking of dynamic changes in RBP-associated RNA profiles under stress conditions. Fusion to PABPC1 further allows monitoring of transcriptomic responses and selectively profile cell-type-specific transcriptomes from mixed populations without cell dissociation and sorting. Additionally, POND-seq supports functional interrogation of RBP domains and residues involved in RNA association and enables scalable analysis of RBP variants, as demonstrated by a systematic assessment of disease-associated FMR1 mutations. Together, POND-seq provides a versatile and scalable platform for non-destructive and longitudinal analysis of cytoplasmic transcriptome and RBP-associated RNAs.","dates":{"publication":"2026/06/24"},"accession":"GSE293919","cross_references":{"GSM":["GSM8893391","GSM8893392","GSM8893393","GSM8893394","GSM8893395","GSM8893396","GSM8893397","GSM8893398","GSM8893399","GSM8893390","GSM8893380","GSM8893381","GSM8893382","GSM8893383","GSM8893384","GSM8893385","GSM8893386","GSM8893387","GSM8893388","GSM8893389","GSM9541790","GSM9541791","GSM9541792","GSM9541793","GSM9541794","GSM9541795","GSM9541796","GSM9541797","GSM9541798","GSM9541799","GSM9541780","GSM9541781","GSM9541782","GSM9541783","GSM9541784","GSM9541785","GSM9541786","GSM9541787","GSM9541788","GSM9541789","GSM9541770","GSM9541771","GSM9541772","GSM9541773","GSM9541774","GSM9541775","GSM9541776","GSM9541777","GSM9541778","GSM9541779","GSM9541769","GSM9541760","GSM9541761","GSM9541762","GSM8893610","GSM9541763","GSM9541764","GSM8893611","GSM9541765","GSM8893612","GSM9541766","GSM8893613","GSM9541767","GSM9541768","GSM9541758","GSM8893605","GSM9541759","GSM8893606","GSM8893607","GSM8893608","GSM8893609","GSM9541750","GSM9541751","GSM9541752","GSM9541753","GSM8893600","GSM8893601","GSM9541754","GSM9541755","GSM8893602","GSM9541756","GSM8893603","GSM8893604","GSM9541757","GSM9541747","GSM9541748","GSM9541749","GSM9541740","GSM9541741","GSM9541742","GSM9541743","GSM9541744","GSM9541745","GSM9541746","GSM9541736","GSM9541737","GSM9541738","GSM9541739","GSM9541730","GSM9541731","GSM9541732","GSM9541733","GSM9541734","GSM9541735","GSM9541846","GSM9541726","GSM9541847","GSM9541727","GSM9541848","GSM9541728","GSM9541849","GSM9541729","GSM8893530","GSM8893410","GSM8893531","GSM8893411","GSM8893532","GSM8893412","GSM8893533","GSM9541840","GSM9541841","GSM8893413","GSM8893534","GSM9541842","GSM8893535","GSM8893536","GSM9541843","GSM9541844","GSM8893416","GSM8893537","GSM8893538","GSM9541845","GSM8893417","GSM9541835","GSM8893407","GSM8893528","GSM9541836","GSM8893529","GSM9541837","GSM9541838","GSM9541839","GSM8893520","GSM8893521","GSM8893400","GSM8893401","GSM8893522","GSM9541830","GSM8893402","GSM8893523","GSM8893524","GSM9541831","GSM8893403","GSM9541832","GSM8893404","GSM8893525","GSM9541833","GSM8893405","GSM8893526","GSM8893527","GSM9541834","GSM8893406","GSM9541824","GSM8893517","GSM8893518","GSM9541825","GSM8893519","GSM9541826","GSM9541827","GSM9541828","GSM9541829","GSM8893510","GSM8893511","GSM8893512","GSM8893513","GSM9541820","GSM9541821","GSM8893514","GSM9541822","GSM8893515","GSM8893516","GSM9541823","GSM9541813","GSM8893506","GSM8893507","GSM9541814","GSM9541815","GSM8893508","GSM9541816","GSM8893509","GSM9541817","GSM9541818","GSM9541819","GSM8893500","GSM8893501","GSM8893502","GSM9541810","GSM8893503","GSM8893504","GSM9541811","GSM9541812","GSM8893505","GSM9541802","GSM9541803","GSM9541804","GSM9541805","GSM9541806","GSM9541807","GSM9541808","GSM9541809","GSM8893570","GSM8893450","GSM8893571","GSM8893330","GSM8893451","GSM8893572","GSM8893573","GSM8893331","GSM8893452","GSM8893332","GSM8893453","GSM8893574","GSM8893333","GSM8893454","GSM8893575","GSM8893455","GSM8893576","GSM8893334","GSM8893335","GSM8893456","GSM8893577","GSM8893578","GSM8893336","GSM8893457","GSM8893458","GSM8893579","GSM8893337","GSM8893338","GSM8893459","GSM8893339","GSM9541800","GSM9541801","GSM8893560","GSM8893561","GSM8893440","GSM8893441","GSM8893562","GSM8893320","GSM8893321","GSM8893442","GSM8893563","GSM8893564","GSM8893322","GSM8893443","GSM8893444","GSM8893565","GSM8893323","GSM8893324","GSM8893445","GSM8893566","GSM8893567","GSM8893325","GSM8893446","GSM8893447","GSM8893568","GSM8893326","GSM8893327","GSM8893448","GSM8893569","GSM8893328","GSM8893449","GSM8893329","GSM8893319","GSM8893550","GSM8893430","GSM8893551","GSM8893310","GSM8893431","GSM8893552","GSM8893553","GSM8893311","GSM8893432","GSM8893312","GSM8893433","GSM8893554","GSM8893313","GSM8893434","GSM8893555","GSM8893435","GSM8893556","GSM8893314","GSM8893315","GSM8893436","GSM8893557","GSM8893558","GSM8893316","GSM8893437","GSM8893438","GSM8893559","GSM8893317","GSM8893318","GSM8893439","GSM8893429","GSM8893540","GSM8893541","GSM8893420","GSM8893421","GSM8893542","GSM8893422","GSM8893543","GSM8893544","GSM8893423","GSM8893424","GSM8893545","GSM8893425","GSM8893546","GSM8893547","GSM8893426","GSM8893427","GSM8893548","GSM8893428","GSM8893549","GSM8893418","GSM8893539","GSM8893419","GSM8893490","GSM8893370","GSM8893491","GSM8893371","GSM8893492","GSM8893372","GSM8893493","GSM8893373","GSM8893494","GSM8893495","GSM8893374","GSM8893375","GSM8893496","GSM8893376","GSM8893497","GSM8893498","GSM8893377","GSM8893378","GSM8893499","GSM8893379","GSM8893480","GSM8893481","GSM8893360","GSM8893361","GSM8893482","GSM8893362","GSM8893483","GSM8893484","GSM8893363","GSM8893364","GSM8893485","GSM8893365","GSM8893486","GSM8893487","GSM8893366","GSM8893367","GSM8893488","GSM8893368","GSM8893489","GSM8893369","GSM8893590","GSM8893470","GSM8893591","GSM8893350","GSM8893471","GSM8893592","GSM8893593","GSM8893351","GSM8893472","GSM8893352","GSM8893473","GSM8893594","GSM8893353","GSM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