{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE293nnn/GSE293961/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Other"],"species":["Mus musculus"],"gds_type":["Other"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE293961"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Limb cell-fate commissioning is guided by widespread coordinated genome detachment from the nuclear lamina [scDam&T-seq]","description":"Diverse forms of heterochromatin block inappropriate transcription and safeguard differentiation and cell identity. Yet, how and when heterochromatin is reconfigured to facilitate changes in cell-fate remains a key open question. Here, we address this by mapping a prevalent heterochromatic feature - genome-lamina interactions - relative to transcription in single-cells during mouse embryogenesis. We find that lamina-genome interactions remain relatively uniform following gastrulation but are extensively reconfigured during organogenesis in diverse tissues. Focusing on limb development, we demonstrate that genome-lamina interactions are selectively released at key developmental genes and their surrounding regulatory domains in early multipotent progenitors. Strikingly, this “lamina-release” often precedes later gene expression, suggesting it primes regulatory domains for future potential activation. Lamina-release also coincides with the putative binding of crucial limb transcription factors and so is closely intertwined with the regulatory machinery that drives limb formation. Conversely, we show that CTCF-defined boundaries of topologically-associated domains (TADs) constrains the spread of lamina-release at a limb gene locus. This ensures independent heterochromatin dynamics between neighbouring domains. Together, this suggests a previously unrecognised process where genome-lamina interactions are selectively dismantled at regulatory domains to transition loci toward more permissive chromatin states, thereby potentiating cell-type specific activation. Our work thus reveals how systematic heterochromatin reorganization links to developmental multipotency, providing mechanistic insight into how progenitors traverse diverse cell-fates in vivo.","dates":{"publication":"2026/07/15"},"accession":"GSE293961","cross_references":{"GSM":["GSM8895272","GSM8895231","GSM8895230","GSM8895274","GSM8895233","GSM8895232","GSM8895276","GSM8895235","GSM8895279","GSM8895278","GSM8895234","GSM8895237","GSM8895236","GSM8895239","GSM8895238","GSM8895271","GSM8895270","GSM8895262","GSM8895261","GSM8895264","GSM8895220","GSM8895263","GSM8895266","GSM8895222","GSM8895221","GSM8895265","GSM8895224","GSM8895268","GSM8895267","GSM8895223","GSM8895226","GSM8895269","GSM8895225","GSM8895228","GSM8895227","GSM8895229","GSM8895260","GSM8895251","GSM8895250","GSM8895294","GSM8895253","GSM8895252","GSM8895255","GSM8895254","GSM8895213","GSM8895257","GSM8895256","GSM8895215","GSM8895259","GSM8895258","GSM8895214","GSM8895217","GSM8895216","GSM8895219","GSM8895218","GSM8895291","GSM8895290","GSM8895293","GSM8895284","GSM8895240","GSM8895283","GSM8895286","GSM8895242","GSM8895241","GSM8895244","GSM8895288","GSM8895243","GSM8895246","GSM8895245","GSM8895248","GSM8895247","GSM8895249","GSM8895281"],"GPL":["19057","24247"],"GSE":["293961"],"taxon":["Mus musculus"]}}