{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE294nnn/GSE294004/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Homo sapiens"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE294004"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Effects of Human Plasma-Like Medium on hPSC-derived cardiomyocytes in vitro","description":"Maturing hPSC-CMs in vitro is critical for advancing drug discovery and cardiotoxicity screening. However, widely used basal medium such as RPMI contain non-physiological compositions that limit their relevance to human cardiac physiology. In this study we introduced Human Plasma-Like Medium (HPLM) as a better basal medium supplemented with B27, a physiologically tailored alternative designed to systematically replicate the salt concentrations and polar metabolite profiles of adult human plasma. Starting with Day16 hPSC-CMs, we cultured cells for two weeks in HPLM/B27 or control media (standard and low-glucose [5 mM] RPMI/B27) and assessed maturation outcomes at Day30. Compared to standard and low-glucose RPMI/B27, HPLM/B27 significantly enhanced hPSC-CM maturity across transcriptomic, structural, functional, and metabolic aspects. These improvements included accomplished myosin heavy chain isoform switching (MYH6 to MYH7), accelerated ventricular-specific myosin light chain isoform switching (MLC2a to MLC2v), elongated sarcomeres (~2.07 µm), enhanced calcium transient kinetics, and coordinated activation of oxidative and glycolytic metabolism. Collectively, these findings establish HPLM as a better basal medium than low glucose RPMI for driving cardiomyocyte maturation in vitro.","dates":{"publication":"2026/04/02"},"accession":"GSE294004","cross_references":{"GSM":["GSM8896241","GSM8896240","GSM8896243","GSM8896242","GSM8896234","GSM8896245","GSM8896244","GSM8896233","GSM8896247","GSM8896236","GSM8896246","GSM8896235","GSM8896238","GSM8896237","GSM8896248","GSM8896239"],"GPL":["24676"],"GSE":["294004"],"taxon":["Homo sapiens"]}}