GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE294nnn/GSE294090/primaryOK200TranscriptomicsDanio rerioExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE294090GEOGSEfalseLoss-of-function mutations in the gene encoding PTH4 cause change of mineral homeostasis, distorted bone development and altered transcription in the kidney of teleost fishPth4 is an ancient parathyroid hormone synthesized by hypothalamic neurons and lost in eutherian mammals. To investigate its role in mineral homeostasis and skeletal development, we generated a pth4 knockout (pth4ko) zebrafish strain using CRISPR/Cas9 and performed transcriptomic analysis across six key tissues: brain, kidney, intestine, gills, scales, and bone. The loss of Pth4 resulted in significant disruptions in calcium and phosphate balance, skeletal abnormalities, and tissue-specific transcriptional changes. Dysregulation of mineral homeostasis genes such as fgf23, phex, and slc34a1a, was observed, particularly in the kidney. Additionally, genes encoding extracellular matrix components (col1a1a, col10a1a, col11a1) and matrix metalloproteinases (mmp9, mmp13a, mmp2) were also differentially expressed in bone, indicating altered bone and scale remodeling. These findings suggest that Pth4 participates in regulating calcium and phosphate balance and bone health in zebrafish possibly through interactions with the FGF23-Klotho axis, thereby contributing to a more comprehensive understanding of the mechanisms underlying mineral regulation and the roles of the PTH family in vertebrate skeletal development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 rerio