GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE294nnn/GSE294300/primaryOK200TranscriptomicsHomo sapiensExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE294300GEOGSEfalseSingle-cell gene expression profiles of cells from primary colorectal cancer and adjacent normal tissue [scRNA-Seq]Colorectal cancer (CRC) is the third malignancy worldwide. RAS mutant CRC has a worse prognosis and resistant to immune therapies. Current research on the tumor microenvironment of RAS-mutant CRC remains limited, with few studies systematically characterizing its cellular composition or functional dynamics. A total of 36 clinical surgical samples (tumors and paired adjacent normal tissues) from 18 patients with primary CRC were collected for single-cell transcriptome sequencing.At the same time, each patient underwent genome sequencing, and 18 patients were divided into RAS gene mutation group (n=10) and wild-type group (n=8). Therefore, revealing the functional cell types and mechanism of immune evasion in RAS mutant CRC by scRNA-seq may contribute to discover new therapeutic targets.2026/07/22GSE294300GSM8901921GSM8901943GSM8901942GSM8901920GSM8901945GSM8901923GSM8901944GSM8901922GSM8901941GSM8901940GSM8901929GSM8901928GSM8901947GSM8901925GSM8901924GSM8901946GSM8901927GSM8901949GSM8901948GSM8901926GSM8901932GSM8901954GSM8901953GSM8901931GSM8901934GSM8901955GSM8901933GSM8901950GSM8901930GSM8901952GSM8901951GSM8901939GSM8901936GSM8901935GSM8901938GSM890193718573294300Homo sapiens[42143353]