GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE294nnn/GSE294338/primaryOK200TranscriptomicsOncorhynchus mykissExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE294338GEOGSEfalseInhibition of gill and skin damage by infectious hematopoietic cell necrosis virus in rainbow trout (Oncorhynchus mykiss) by astragalus polysaccharides through promotion of inactivated vaccine efficacyWith a growing population and increased awareness of the benefits of consuming fish products, meeting the increasing demand for fish products has become critical to aquaculture activities. However, Infectious Hematopoietic Organ Necrosis Virus (IHNV) causes Infectious Hematopoietic Organ Necrosis (IHN) disease, which in severe cases may lead to 100% mortality in juvenile rainbow trout, and has become a problematic issue in the development of aquaculture.IHNV leads to degenerative necrosis of hematopoietic tissues, and the symptoms, including darkening of the skin color, protrusion of the eyes, pallor of the gills, and distension of the abdomen, pose the greatest threat in the juvenile stage of the fish. Gills and skin are mucosal tissues of fish that are in direct contact with the aquatic environment and are key sites of invasion for a variety of pathogens. Here, we investigated the effect of Astragalus polysaccharide combined with inactivated IHNV vaccine (APS+V) on gill and skin immunity in rainbow trout. Histopathological results showed that APS+V significantly inhibited IHNV-induced skin and gill damage and apoptosis. Astragalus polysaccharide also increased tissue SOD, CAT, and T-AOC activities by enhancing vaccine efficacy. Transcriptome analysis showed that immune-related pathways were significantly enriched in the APS + vaccine group. Key immune genes, including IgM, IgT, IgD, CD4, CD8; APS + vaccine induced the expression of these genes in tissues. Notably, APS + V significantly inhibited IHNV replication in mucosal tissues.2026/05/01GSE294338GSM8902651GSM8902652GSM8902630GSM8902650GSM8902637GSM8902638GSM8902657GSM8902635GSM8902658GSM8902636GSM8902655GSM8902633GSM8902634GSM8902656GSM8902631GSM8902653GSM8902654GSM8902632GSM8902639GSM8902640GSM8902641GSM8902648GSM8902626GSM8902649GSM8902627GSM8902646GSM8902624GSM8902625GSM8902647GSM8902644GSM8902645GSM8902623GSM8902642GSM8902643GSM8902628GSM890262929994294338Oncorhynchus mykiss