GEOOtherHomo sapiensOtherhttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE294385GEOGSEfalseSpatial Multi-Omics Landscape of Colorectal Cancer Macro- and MicrometastasesColorectal cancer (CRC) metastases often recur due to minimal residual disease (MRD) and micrometastases that persist after therapy. Here, we analyzed 341,328 spatially resolved spots from 37 tumors, including liver (CLiM) and lung (CLuM) metastases, and primary CRC from 11 patients. Phylogenetic analysis of copy number variations revealed heterogeneous tumor evolution, with early dissemination and quiescence in liver micrometastases (CLiMi). Spatial analysis identified 10 conserved and 7 distinct metaprograms, highlighting stromal interactions in CLiM and CLuM, with macrophages enriched in CLiM and lymphocytes in CLuM. Micrometastases showed strong immunosuppressive signature and increased T cell exhaustion. Notably, we identified a CLiMi-specific six-gene signature associated with MRD status, disease-free survival, and chemotherapy resistance across multiple independent cohorts. These findings elucidate the spatial evolutionary landscape of CRC metastases and provide tissue-based spatially validated biomarkers for surveillance and therapeutic targeting.2026/06/02GSE294385GSM8903685GSM8903663GSM8903664GSM8903686GSM8903661GSM8903683GSM8903684GSM8903662GSM8903681GSM8903682GSM8903660GSM8903680GSM8903669GSM8903689GSM8903667GSM8903668GSM8903687GSM8903665GSM8903688GSM8903666GSM8903674GSM8903675GSM8903672GSM8903673GSM8903692GSM8903670GSM8903693GSM8903671GSM8903690GSM8903691GSM8903658GSM8903659GSM8903678GSM8903679GSM8903657GSM8903676GSM890367724676294385Homo sapiens