GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE294nnn/GSE294441/primaryOK200TranscriptomicsHomo sapiensExpression profiling by arrayhttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE294441GEOGSEfalseNanostring-Based Gene Expression Profiling of Melanoma Lymph Node MetastasesThis study investigates gene expression profiles in metastatic melanoma lymph node samples using the Nanostring nCounter platform. Samples were obtained from patients who were treatment-naïve, resistant, or responsive to immune checkpoint inhibitor (ICI) therapy. We applied both the PanCancer Immune Profiling Panel and a custom tissue-resident memory (TRM) T cell panel to assess immune cell composition and activation states. The dataset reveals transcriptional signatures associated with ICI response, including enrichment of TRM-associated genes and interferon-stimulated pathways in responders, and immunosuppressive features in resistant tumors. These data provide insights into the role of tumour-resident T cells and the tumour immune microenvironment in shaping response to immunotherapy.2026/06/08GSE294441GSM8905372GSM8905361GSM8905371GSM8905360GSM8905370GSM8905369GSM8905358GSM8905357GSM8905379GSM8905368GSM8905378GSM8905367GSM8905356GSM8905377GSM8905366GSM8905365GSM8905376GSM8905364GSM8905375GSM8905374GSM8905363GSM8905373GSM8905362GSM890535935751294441Homo sapiens