GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE294nnn/GSE294535/primaryOK200TranscriptomicsMus musculusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE294535GEOGSEfalseModular neuroinflammatory network discovery from large-scale phenotypic screening in genetically heterogeneous mice[part5]The central nervous system represents a uniquely immune-privileged environment, with inflammatory responses involving several resident CNS-specific cell types. While stereotyped cellular and transcriptional responses recur across varied diseases, relevant signaling pathways and regulatory networks are not fully understood. Here, we describe a screening platform based on high-throughput RNA-seq analysis and glial cell state gene set scores that enables multimodal investigation of inflammatory networks at scale. As proof-of-concept, we investigate genetically heterogeneous mice from a large-scale chemical mutagenesis screen to identify novel functionally relevant variants in six genes previously linked to human CNS disorders: Nrros, Ctsd, Smpd1, Idua, Nlrp1a, and Inpp5d . We leverage the rich and readily-interpretable data from our study to demarcate distinct inflammatory states arising from each mutation. In all, our work provides a methodology for discovering novel regulators of CNS neuroimmune homeostasis and provides a validated analysis framework for identifying discrete neuroinflammatory modules that are engaged divergently across disease contexts.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M8909893GSM8910409GSM890989224247294535Mus musculus