GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE294nnn/GSE294643/primaryOK200TranscriptomicsMus musculusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE294643GEOGSEfalseModular neuroinflammatory network discovery from large-scale phenotypic screening in genetically heterogeneous mice [part6]The central nervous system represents a uniquely immune-privileged environment, with inflammatory responses involving several resident CNS-specific cell types. While stereotyped cellular and transcriptional responses recur across varied diseases, relevant signaling pathways and regulatory networks are not fully understood. Here, we describe a screening platform based on high-throughput RNA-seq analysis and glial cell state gene set scores that enables multimodal investigation of inflammatory networks at scale. As proof-of-concept, we investigate genetically heterogeneous mice from a large-scale chemical mutagenesis screen to identify novel functionally relevant variants in six genes previously linked to human CNS disorders: Nrros, Ctsd, Smpd1, Idua, Nlrp1a, and Inpp5d . We leverage the rich and readily-interpretable data from our study to demarcate distinct inflammatory states arising from each mutation. In all, our work provides a methodology for discovering novel regulators of CNS neuroimmune homeostasis and provides a validated analysis framework for identifying discrete neuroinflammatory modules that are engaged divergently across disease contexts.2026/04/11GSE294643GSM8913290GSM8913291GSM8913292GSM8913297GSM8913298GSM8913299GSM8913293GSM8913294GSM8913295GSM8913296GSM8914119GSM8914115GSM8913268GSM8914116GSM8913269GSM8914117GSM8914118GSM8914111GSM8913264GSM8914112GSM8913265GSM8913266GSM8914113GSM8914114GSM8913267GSM8913260GSM8913261GSM8913262GSM8913263GSM8914110GSM8914108GSM8914109GSM8913499GSM8914104GSM8913257GSM8914105GSM8913258GSM8913259GSM8914106GSM8914107GSM8913490GSM8913495GSM8913253GSM8914100GSM8913496GSM8914101GSM8913254GSM8914102GSM8913255GSM8913497GSM8913498GSM8914103GSM8913256GSM8913491GSM8913492GSM8913250GSM8913493GSM8913251GSM8913252GSM8913494GSM8913280GSM8913281GSM8913286GSM8913287GSM8913288GSM8913289GSM8913282GSM8913283GSM8913284GSM8913285GSM8913279GSM8914126GSM8914127GSM8913270GSM8913275GSM8914122GSM8914123GSM8913276GSM8914124GSM8913277GSM8914125GSM8913278GSM8913271GSM8913272GSM8914120GSM8913273GSM8914121GSM8913274GSM8913228GSM8913229GSM8913224GSM8913466GSM8913467GSM8913225GSM8913226GSM8913468GSM8913469GSM8913227GSM8913220GSM8913462GSM8913221GSM8913463GSM8913464GSM8913222GSM8913223GSM8913465GSM8913460GSM8913461GSM8913459GSM8913217GSM8913218GSM8913219GSM8913455GSM8913213GSM8913697GSM8913698GSM8913456GSM8913214GSM8913215GSM8913699GSM8913457GSM8913458GSM8913216GSM8913693GSM8913451GSM8913452GSM8913694GSM8913210GSM8913453GSM8913211GSM8913695GSM8913212GSM8913696GSM8913454GSM8913690GSM8913691GSM8913450GSM8913692GSM8913246GSM8913488GSM8913247GSM8913489GSM8913248GSM8913249GSM8913484GSM8913242GSM8913243GSM8913485GSM8913244GSM8913486GSM8913487GSM8913245GSM8913480GSM8913481GSM8913482GSM8913240GSM8913241GSM8913483GSM8913239GSM8913235GSM8913477GSM8913478GSM8913236GSM8913479GSM8913237GSM8913238GSM8913473GSM8913231GSM8913232GSM8913474GSM8913475GSM8913233GSM8913476GSM8913234GSM8913470GSM8913471GSM8913472GSM8913230GSM8913668GSM8913426GSM8913427GSM8913669GSM8913428GSM8913429GSM8913664GSM8913422GSM8913665GSM8913423GSM8913424GSM8913666GSM8913667GSM8913425GSM8913660GSM8913661GSM8913662GSM8913420GSM8913421GSM8913663GSM8913899GSM8913415GSM8913657GSM8913658GSM8913416GSM8913659GSM8913417GSM8913418GSM8913653GSM8913411GSM8913895GSM8913412GSM8913896GSM8913654GSM8913413GSM8913897GSM8913655GSM8913656GSM8913898GSM8913414GSM8913419GSM8913891GSM8913650GSM8913892GSM8913893GSM8913651GSM8913410GSM8913894GSM8913652GSM8913890GSM8913206GSM8913448GSM8913449GSM8913207GSM8913208GSM8913209GSM8913444GSM8913202GSM8913686GSM8913203GSM8913687GSM8913445GSM8913204GSM8913688GSM8913446GSM8913447GSM8913205GSM8913689GSM8913682GSM8913440GSM8913441GSM8913683GSM8913684GSM8913200GSM8913442GSM8913201GSM8913685GSM8913443GSM8913680GSM8913681GSM8913679GSM8913437GSM8913438GSM8913439GSM8913675GSM8913676GSM8913677GSM8913678GSM8913436GSM8913671GSM8913672GSM8913673GSM8913674GSM8913670GSM8913624GSM8913866GSM8913867GSM8913625GSM8913868GSM8913626GSM8913627GSM8913869GSM8913862GSM8913620GSM8913621GSM8913863GSM8913622GSM8913864GSM8913865GSM8913623GSM8913628GSM8913629GSM8913860GSM8913861GSM8913613GSM8913855GSM8913856GSM8913614GSM8913615GSM8913857GSM8913616GSM8913858GSM8913851GSM8913610GSM8913852GSM8913853GSM8913611GSM8913854GSM8913612GSM8913859GSM8913617GSM8913618GSM8913619GSM8913850GSM8913404GSM8913888GSM8913646GSM8913647GSM8913889GSM8913405GSM8913648GSM8913406GSM8913407GSM8913649GSM8913642GSM8913400GSM8913884GSM8913401GSM8913885GSM8913643GSM8913644GSM8913886GSM8913402GSM8913887GSM8913645GSM8913403GSM8913408GSM8913409GSM8913880GSM8913881GSM8913882GSM8913640GSM8913641GSM8913883GSM8913877GSM8913635GSM8913636GSM8913878GSM8913879GSM8913637GSM8913638GSM8913873GSM8913631GSM8913874GSM8913632GSM8913633GSM8913875GSM8913876GSM8913634GSM8913639GSM8913870GSM8913871GSM8913630GSM8913872GSM8913822GSM8913824GSM8913826GSM8913827GSM8913828GSM8913829GSM8913811GSM8913813GSM8913819GSM8913815GSM8913817GSM8913844GSM8913602GSM8913845GSM8913603GSM8913604GSM8913846GSM8913847GSM8913605GSM8913840GSM8913841GSM8913842GSM8913600GSM8913601GSM8913843GSM8913848GSM8913606GSM8913607GSM8913849GSM8913608GSM8913609GSM8913833GSM8913834GSM8913835GSM8913836GSM8913830GSM8913831GSM8913832GSM8913837GSM8913838GSM8913839GSM8913800GSM8913801GSM8913802GSM8913803GSM8913809GSM8913805GSM8913807GSM8914070GSM8914071GSM8914072GSM8914073GSM8914078GSM8914079GSM8914074GSM8914075GSM8914076GSM8914077GSM8914060GSM8914061GSM8914062GSM8914067GSM8914068GSM8914069GSM8914063GSM8914064GSM8914065GSM8914066GSM8914092GSM8914093GSM8914094GSM8914095GSM8914090GSM8914091GSM8914096GSM8914097GSM8914098GSM8914099GSM8914081GSM8914082GSM8914083GSM8914084GSM8914080GSM8914089GSM8914085GSM8914086GSM8914087GSM8914088GSM8914038GSM8914039GSM8913180GSM8913181GSM8913182GSM8914034GSM8913187GSM8914035GSM8913188GSM8914036GSM8913189GSM8914037GSM8913183GSM8914030GSM8914031GSM8913184GSM8913185GSM8914032GSM8914033GSM8913186GSM8914027GSM8914028GSM8914029GSM8913170GSM8913171GSM8913176GSM8914023GSM8914024GSM8913177GSM8914025GSM8913178GSM8913179GSM8914026GSM8913172GSM8914020GSM8913173GSM8914021GSM8913174GSM8914022GSM8914050GSM8914051GSM8914056GSM8914057GSM8914058GSM8914059GSM8914052GSM8914053GS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M8913915GSM8913916GSM891391724247294643Mus musculus