GEOTranscriptomicsMus musculusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE294665GEOGSEfalseModular neuroinflammatory network discovery from large-scale phenotypic screening in genetically heterogeneous mice [part7]The central nervous system represents a uniquely immune-privileged environment, with inflammatory responses involving several resident CNS-specific cell types. While stereotyped cellular and transcriptional responses recur across varied diseases, relevant signaling pathways and regulatory networks are not fully understood. Here, we describe a screening platform based on high-throughput RNA-seq analysis and glial cell state gene set scores that enables multimodal investigation of inflammatory networks at scale. As proof-of-concept, we investigate genetically heterogeneous mice from a large-scale chemical mutagenesis screen to identify novel functionally relevant variants in six genes previously linked to human CNS disorders: Nrros, Ctsd, Smpd1, Idua, Nlrp1a, and Inpp5d . We leverage the rich and readily-interpretable data from our study to demarcate distinct inflammatory states arising from each mutation. In all, our work provides a methodology for discovering novel regulators of CNS neuroimmune homeostasis and provides a validated analysis framework for identifying discrete neuroinflammatory modules that are engaged divergently across disease contexts.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M8915098GSM8915097GSM891509924247294665Mus musculus