GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE294nnn/GSE294686/primaryOK200TranscriptomicsMus musculusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE294686GEOGSEfalseModular neuroinflammatory network discovery from large-scale phenotypic screening in genetically heterogeneous mice [part8]The central nervous system represents a uniquely immune-privileged environment, with inflammatory responses involving several resident CNS-specific cell types. While stereotyped cellular and transcriptional responses recur across varied diseases, relevant signaling pathways and regulatory networks are not fully understood. Here, we describe a screening platform based on high-throughput RNA-seq analysis and glial cell state gene set scores that enables multimodal investigation of inflammatory networks at scale. As proof-of-concept, we investigate genetically heterogeneous mice from a large-scale chemical mutagenesis screen to identify novel functionally relevant variants in six genes previously linked to human CNS disorders: Nrros, Ctsd, Smpd1, Idua, Nlrp1a, and Inpp5d . We leverage the rich and readily-interpretable data from our study to demarcate distinct inflammatory states arising from each mutation. In all, our work provides a methodology for discovering novel regulators of CNS neuroimmune homeostasis and provides a validated analysis framework for identifying discrete neuroinflammatory modules that are engaged divergently across disease contexts.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M8916186GSM8916189GSM891618824247294686Mus musculus