GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE294nnn/GSE294826/primaryOK200TranscriptomicsMus musculusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE294826GEOGSEfalseModular neuroinflammatory network discovery from large-scale phenotypic screening in genetically heterogeneous mice [part9]The central nervous system represents a uniquely immune-privileged environment, with inflammatory responses involving several resident CNS-specific cell types. While stereotyped cellular and transcriptional responses recur across varied diseases, relevant signaling pathways and regulatory networks are not fully understood. Here, we describe a screening platform based on high-throughput RNA-seq analysis and glial cell state gene set scores that enables multimodal investigation of inflammatory networks at scale. As proof-of-concept, we investigate genetically heterogeneous mice from a large-scale chemical mutagenesis screen to identify novel functionally relevant variants in six genes previously linked to human CNS disorders: Nrros, Ctsd, Smpd1, Idua, Nlrp1a, and Inpp5d . We leverage the rich and readily-interpretable data from our study to demarcate distinct inflammatory states arising from each mutation. In all, our work provides a methodology for discovering novel regulators of CNS neuroimmune homeostasis and provides a validated analysis framework for identifying discrete neuroinflammatory modules that are engaged divergently across disease contexts.2026/04/11GSE294826GSM8921970GSM8921972GSM8921971GSM8921974GSM8922822GSM8922823GSM8921973GSM8921976GSM8922820GSM8922821GSM8921975GSM8921978GSM8922826GSM8921977GSM8922827GSM8922824GSM8922825GSM8921979GSM8922828GSM8922829GSM8921981GSM8921980GSM8922830GSM8921983GSM8921982GSM8922833GSM8921985GSM8922834GSM8921984GSM8922831GSM8921987GSM8921986GSM8922832GSM8921989GSM8922837GSM8921988GSM8922838GSM8922835GSM8922836GSM8922839GSM8921950GSM8921952GSM8922800GSM8921951GSM8922801GSM8921954GSM8921953GSM8922804GSM8921956GSM8922805GSM8921955GSM8922802GSM8921958GSM8921957GSM8922803GSM8922808GSM8922809GSM8921959GSM8922806GSM8922807GSM8921961GSM8921960GSM8921963GSM8922811GSM8922812GSM8921962GSM8921965GSM8922810GSM8921964GSM8921967GSM8922815GSM8922816GSM8921966GSM8922813GSM8921969GSM8921968GSM8922814GSM8922819GSM8922817GSM8922818GSM8921930GSM8921932GSM8921931GSM8921934GSM8921933GSM8921936GSM8921935GSM8921938GSM8921937GSM8921939GSM8921941GSM8921940GSM8921943GSM8921942GSM8921945GSM8921944GSM8921947GSM8921946GSM8921949GSM8921948GSM8921909GSM8921908GSM8921910GSM8921912GSM8921911GSM8921914GSM8921913GSM8921916GSM8921915GSM8921918GSM8921917GSM8921919GSM8921921GSM8921920GSM8921923GSM8921922GSM8921925GSM8921924GSM8921927GSM8921926GSM8921929GSM8921928GSM8921901GSM8921900GSM8921903GSM8921902GSM8921905GSM8921904GSM8921907GSM8921906GSM8922180GSM8922183GSM8922184GSM8922181GSM8922182GSM8922187GSM8922188GSM8922185GSM8922186GSM8922189GSM8922190GSM8922191GSM8922194GSM8922195GSM8922192GSM8922193GSM8922198GSM8922199GSM8922196GSM8922197GSM8922161GSM8922162GSM8922160GSM8922165GSM8922166GSM8922163GSM8922164GSM8922169GSM8922167GSM8922168GSM8922172GSM8922173GSM8922170GSM8922171GSM8922176GSM8922177GSM8922174GSM8922175GSM8922178GSM8922179GSM8922381GSM8922140GSM8922382GSM8922380GSM8922143GSM8922385GSM8922386GSM8922144GSM8922383GSM8922141GSM8922384GSM8922142GSM8922389GSM8922147GSM8922148GSM8922387GSM8922145GSM8922146GSM8922388GSM8922149GSM8922392GSM8922150GSM8922393GSM8922151GSM8922390GSM8922391GSM8922154GSM8922396GSM8922155GSM8922397GSM8922152GSM8922394GSM8922395GSM8922153GSM8922158GSM8922159GSM8922398GSM8922156GSM8922157GSM8922399GSM8922360GSM8922363GSM8922121GSM8922364GSM8922122GSM8922361GSM8922120GSM8922362GSM8922367GSM8922125GSM8922126GSM8922368GSM8922123GSM8922365GSM8922366GSM8922124GSM8922129GSM8922369GSM8922127GSM8922128GSM8922370GSM8922371GSM8922132GSM8922374GSM8922375GSM8922133GSM8922372GSM8922130GSM8922131GSM8922373GSM8922378GSM8922136GSM8922137GSM8922379GSM8922134GSM8922376GSM8922135GSM8922377GSM8922138GSM8922139GSM8922580GSM8922341GSM8922583GSM8922100GSM8922584GSM8922581GSM8922340GSM8922582GSM8922103GSM8922587GSM8922346GSM8922104GSM8922588GSM8922585GSM8922101GSM8922102GSM8922586GSM8922107GSM8922108GSM8922589GSM8922105GSM8922106GSM8922109GSM8922590GSM8922591GSM8922352GSM8922594GSM8922110GSM8922595GSM8922111GSM8922353GSM8922592GSM8922350GSM8922593GSM8922114GSM8922598GSM8922356GSM8922115GSM8922599GSM8922357GSM8922112GSM8922596GSM8922354GSM8922355GSM8922113GSM8922597GSM8922118GSM8922119GSM8922358GSM8922116GSM8922117GSM8922359GSM8922561GSM8922320GSM8922562GSM8922560GSM8922323GSM8922565GSM8922324GSM8922566GSM8922321GSM8922563GSM8922564GSM8922322GSM8922569GSM8922327GSM8922328GSM8922567GSM8922325GSM8922326GSM8922568GSM8922329GSM8922572GSM8922330GSM8922573GSM8922331GSM8922570GSM8922571GSM8922576GSM8922334GSM8922335GSM8922577GSM8922332GSM8922574GSM8922575GSM8922333GSM8922338GSM8922339GSM8922578GSM8922336GSM8922579GSM8922337GSM8922782GSM8922540GSM8922301GSM8922543GSM8922544GSM8922302GSM8922541GSM8922300GSM8922542GSM8922547GSM8922789GSM8922305GSM8922306GSM8922548GSM8922303GSM8922787GSM8922545GSM8922304GSM8922788GSM8922546GSM8922309GSM8922549GSM8922307GSM8922308GSM8922550GSM8922792GSM8922793GSM8922551GSM8922790GSM8922791GSM8922312GSM8922796GSM8922554GSM8922555GSM8922313GSM8922797GSM8922552GSM8922310GSM8922794GSM8922553GSM8922311GSM8922795GSM8922558GSM8922316GSM8922559GSM8922317GSM8922798GSM8922556GSM8922314GSM8922799GSM8922315GSM8922557GSM8922318GSM8922319GSM8922760GSM8922521GSM8922763GSM8922764GSM8922522GSM8922761GSM8922762GSM8922520GSM8922767GSM8922525GSM8922768GSM8922526GSM8922765GSM8922523GSM8922524GSM8922766GSM8922529GSM8922527GSM8922769GSM8922528GSM8922770GSM8922771GSM8922532GSM8922774GSM8922533GSM8922530GSM8922531GSM8922778GSM8922536GSM8922537GSM8922534GSM8922535GSM8922538GSM8922539GSM8921891GSM8921890GSM8922741GSM8921893GSM8922742GSM8921892GSM8922500GSM8921895GSM8921894GSM8922740GSM8921897GSM8922745GSM8922503GSM8922504GSM8921896GSM8922746GSM8922501GSM8922743GSM8921899GSM8922744GSM8922502GSM8921898GSM8922507GSM8922749GSM8922508GSM8922747GSM8922505GSM8922506GSM8922748GSM8922509GSM8922510GSM8922752GSM8922753GSM8922511GSM8922750GSM8922751GSM8922756GSM8922514GSM8922515GSM8922757GSM8922512GSM8922754GSM8922513GSM8922755GSM8922518GSM8922519GSM8922758GSM8922516GSM8922759GSM8922517GSM8921871GSM8922720GSM8921870GSM8921873GSM8921872GSM8921875GSM8922723GSM8922724GSM8921874GSM8922721GSM8921877GSM8922722GSM8921876GSM8921879GSM8921878GSM8921880GSM8921882GSM8921881GSM8921884GSM8921883GSM8921886GSM8921885GSM8921888GSM8922733GSM8921887GSM8922738GSM8921889GSM8922739GSM8921851GSM8921850GSM8922701GSM8921853GSM8922702GSM8921852GSM8921855GSM8921854GSM8922700GSM8921857GSM8922705GSM8922706GSM8921856GSM8922703GSM8921859GSM8922704GSM8921858GSM8922709GSM8922707GSM8922708GSM8921860GSM8921862GSM8921861GSM8921864GSM8922712GSM8922713GSM8921863GSM8922710GSM8921866GSM8921865GSM8922711GSM8921868GSM8922716GSM8922717GSM8921867GSM8922714GSM8922715GSM8921869GSM8922718GSM8922719GSM8921835GSM8921840GSM8921842GSM8921841GSM8921844GSM8921843GSM8921846GSM8921845GSM8921848GSM8921847GSM8921849GSM8921809GSM8921811GSM8921810GSM8921813GSM8921812GSM8921815GSM8921814GSM8921817GSM8921816GSM8921819GSM8921818GSM8921820GSM8921822GSM8921821GSM8921826GSM8921800GSM8921802GSM8921801GSM8921804GSM8921803GSM8921806GSM8921805GSM8921808GSM8921807GSM8922080GSM8922081GSM8922084GSM8922085GSM8922082GSM8922083GSM8922088GSM8922089GSM8922086GSM8922087GSM8922091GSM8922092GSM8922090GSM8922095GSM8922096GSM8922093GSM8922094GSM8922099GSM8922097GSM8922098GSM8922062GSM8922063GSM8922060GSM8922061GSM8922066GSM8922067GSM8922064GSM8922065GSM8922068GSM8922069GSM8922070GSM8922073GSM8922074GSM8922071GSM8922072GSM8922077GSM8922078GSM8922075GSM8922076GSM8922079GSM8922040GSM8922282GSM8922283GSM8922041GSM8922280GSM8922281GSM8922286GSM8922044GSM8922287GSM8922045GSM8922284GSM8922042GSM8922043GSM8922285GSM8922048GSM8922049GSM8922046GSM8922288GSM8922289GSM8922047GSM8922290GSM8922051GSM8922293GSM8922052GSM8922294GSM8922291GSM8922292GSM8922050GSM8922055GSM8922297GSM8922298GSM8922056GSM8922295GSM8922053GSM8922054GSM8922296GSM8922059GSM8922057GSM8922299GSM8922058GSM8922260GSM8922261GSM8922264GSM8922022GSM8922023GSM8922265GSM8922020GSM8922262GSM8922263GSM8922021GSM8922026GSM8922268GSM8922269GSM8922027GSM8922266GSM8922024GSM8922267GSM8922025GSM8922028GSM8922029GSM8922271GSM8922272GSM8922030GSM8922270GSM8922275GSM8922033GSM8922034GSM8922276GSM8922031GSM8922273GSM8922032GSM8922274GSM8922037GSM8922279GSM8922038GSM8922277GSM8922035GSM8922278GSM8922036GSM8922039GSM8922480GSM8922481GSM8922000GSM8922484GSM8922242GSM8922243GSM8922485GSM8922001GSM8922240GSM8922482GSM8922241GSM8922483GSM8922246GSM8922488GSM8922004GSM8922489GSM8922247GSM8922005GSM8922486GSM8922244GSM8922002GSM8922003GSM8922487GSM8922245GSM8922008GSM8922009GSM8922006GSM8922248GSM8922249GSM8922007GSM8922491GSM8922492GSM8922250GSM8922490GSM8922011GSM8922495GSM8922253GSM8922012GSM8922496GSM8922254GSM8922493GSM8922251GSM8922252GSM8922010GSM8922494GSM8922257GSM8922015GSM8922499GSM8922258GSM8922016GSM8922255GSM8922013GSM8922497GSM8922014GSM8922498GSM8922256GSM8922019GSM8922017GSM8922259GSM8922018GSM8922220GSM8922462GSM8922221GSM8922463GSM8922460GSM8922461GSM8922466GSM8922224GSM8922467GSM8922225GSM8922464GSM8922222GSM8922223GSM8922465GSM8922228GSM8922229GSM8922226GSM8922468GSM8922469GSM8922227GSM8922470GSM8922473GSM8922231GSM8922232GSM8922474GSM8922471GSM8922472GSM8922230GSM8922235GSM8922477GSM8922478GSM8922236GSM8922475GSM8922233GSM8922476GSM8922234GSM8922239GSM8922237GSM8922479GSM8922238GSM8922682GSM8922440GSM8922441GSM8922683GSM8922680GSM8922681GSM8922444GSM8922202GSM8922686GSM8922687GSM8922203GSM8922445GSM8922200GSM8922684GSM8922442GSM8922201GSM8922685GSM8922443GSM8922206GSM8922448GSM8922449GSM8922207GSM8922446GSM8922688GSM8922204GSM8922447GSM8922205GSM8922689GSM8922208GSM8922209GSM8922690GSM8922693GSM8922451GSM8922452GSM8922694GSM8922210GSM8922691GSM8922450GSM8922692GSM8922455GSM8922697GSM8922213GSM8922698GSM8922456GSM8922214GSM8922695GSM8922453GSM8922211GSM8922212GSM8922696GSM8922454GSM8922217GSM8922459GSM8922218GSM8922215GSM8922699GSM8922457GSM8922458GSM8922216GSM8922219GSM8922660GSM8922661GSM8922664GSM8922422GSM8922423GSM8922665GSM8922662GSM8922420GSM8922421GSM8922663GSM8922426GSM8922668GSM8922427GSM8922669GSM8922424GSM8922666GSM8922667GSM8922425GSM8922428GSM8922429GSM8922671GSM8922430GSM8922672GSM8922670GSM8922675GSM8922433GSM8922676GSM8922434GSM8922673GSM8922431GSM8922432GSM8922674GSM8922679GSM8922437GSM8922438GSM8922435GSM8922677GSM8922678GSM8922436GSM8922439GSM8922642GSM8922400GSM8922401GSM8922643GSM8922640GSM8922641GSM8922404GSM8921798GSM8922646GSM8922647GSM8922405GSM8922644GSM8922402GSM8922403GSM8921799GSM8922645GSM8922408GSM8922409GSM8922406GSM8922648GSM8922407GSM8922649GSM8922650GSM8922653GSM8922411GSM8922412GSM8922654GSM8922651GSM8922652GSM8922410GSM8922415GSM8922657GSM8922658GSM8922416GSM8922655GSM8922413GSM8922656GSM8922414GSM8922419GSM8922659GSM8922417GSM8922418GSM8922620GSM8922621GSM8922624GSM8922625GSM8922622GSM8922623GSM8922628GSM8922629GSM8922626GSM8922627GSM8922631GSM8922632GSM8922630GSM8922635GSM8922636GSM8922633GSM8922634GSM8922639GSM8922637GSM8922638GSM8921990GSM8921992GSM8922840GSM8921991GSM8922841GSM8921994GSM8921993GSM8922844GSM8922602GSM8921996GSM8921995GSM8922603GSM8922842GSM8921998GSM8922600GSM8921997GSM8922601GSM8922843GSM8922606GSM8922607GSM8921999GSM8922604GSM8922605GSM8922608GSM8922609GSM8922610GSM8922613GSM8922614GSM8922611GSM8922612GSM8922617GSM8922618GSM8922615GSM8922616GSM892261924247294826Mus musculus