GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE294nnn/GSE294836/primaryOK200TranscriptomicsMus musculusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE294836GEOGSEfalseModular neuroinflammatory network discovery from large-scale phenotypic screening in genetically heterogeneous mice[part11]The central nervous system represents a uniquely immune-privileged environment, with inflammatory responses involving several resident CNS-specific cell types. While stereotyped cellular and transcriptional responses recur across varied diseases, relevant signaling pathways and regulatory networks are not fully understood. Here, we describe a screening platform based on high-throughput RNA-seq analysis and glial cell state gene set scores that enables multimodal investigation of inflammatory networks at scale. As proof-of-concept, we investigate genetically heterogeneous mice from a large-scale chemical mutagenesis screen to identify novel functionally relevant variants in six genes previously linked to human CNS disorders: Nrros, Ctsd, Smpd1, Idua, Nlrp1a, and Inpp5d . We leverage the rich and readily-interpretable data from our study to demarcate distinct inflammatory states arising from each mutation. In all, our work provides a methodology for discovering novel regulators of CNS neuroimmune homeostasis and provides a validated analysis framework for identifying discrete neuroinflammatory modules that are engaged divergently across disease contexts.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M8923949GSM8923946GSM892394724247294836Mus musculus