GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE294nnn/GSE294845/primaryOK200TranscriptomicsMus musculusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE294845GEOGSEfalseModular neuroinflammatory network discovery from large-scale phenotypic screening in genetically heterogeneous mice [part12]The central nervous system represents a uniquely immune-privileged environment, with inflammatory responses involving several resident CNS-specific cell types. While stereotyped cellular and transcriptional responses recur across varied diseases, relevant signaling pathways and regulatory networks are not fully understood. Here, we describe a screening platform based on high-throughput RNA-seq analysis and glial cell state gene set scores that enables multimodal investigation of inflammatory networks at scale. As proof-of-concept, we investigate genetically heterogeneous mice from a large-scale chemical mutagenesis screen to identify novel functionally relevant variants in six genes previously linked to human CNS disorders: Nrros, Ctsd, Smpd1, Idua, Nlrp1a, and Inpp5d . We leverage the rich and readily-interpretable data from our study to demarcate distinct inflammatory states arising from each mutation. In all, our work provides a methodology for discovering novel regulators of CNS neuroimmune homeostasis and provides a validated analysis framework for identifying discrete neuroinflammatory modules that are engaged divergently across disease contexts.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M8925904GSM8925909GSM892590824247294845Mus musculus