GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE294nnn/GSE294849/primaryOK200TranscriptomicsMus musculusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE294849GEOGSEfalseModular neuroinflammatory network discovery from large-scale phenotypic screening in genetically heterogeneous mice [part13]The central nervous system represents a uniquely immune-privileged environment, with inflammatory responses involving several resident CNS-specific cell types. While stereotyped cellular and transcriptional responses recur across varied diseases, relevant signaling pathways and regulatory networks are not fully understood. Here, we describe a screening platform based on high-throughput RNA-seq analysis and glial cell state gene set scores that enables multimodal investigation of inflammatory networks at scale. As proof-of-concept, we investigate genetically heterogeneous mice from a large-scale chemical mutagenesis screen to identify novel functionally relevant variants in six genes previously linked to human CNS disorders: Nrros, Ctsd, Smpd1, Idua, Nlrp1a, and Inpp5d . We leverage the rich and readily-interpretable data from our study to demarcate distinct inflammatory states arising from each mutation. In all, our work provides a methodology for discovering novel regulators of CNS neuroimmune homeostasis and provides a validated analysis framework for identifying discrete neuroinflammatory modules that are engaged divergently across disease contexts.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M8926817GSM8926818GSM892681924247294849Mus musculus