GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE294nnn/GSE294978/primaryOK200TranscriptomicsMus musculusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE294978GEOGSEfalseModular neuroinflammatory network discovery from large-scale phenotypic screening in genetically heterogeneous mice [part19]The central nervous system represents a uniquely immune-privileged environment, with inflammatory responses involving several resident CNS-specific cell types. While stereotyped cellular and transcriptional responses recur across varied diseases, relevant signaling pathways and regulatory networks are not fully understood. Here, we describe a screening platform based on high-throughput RNA-seq analysis and glial cell state gene set scores that enables multimodal investigation of inflammatory networks at scale. As proof-of-concept, we investigate genetically heterogeneous mice from a large-scale chemical mutagenesis screen to identify novel functionally relevant variants in six genes previously linked to human CNS disorders: Nrros, Ctsd, Smpd1, Idua, Nlrp1a, and Inpp5d . We leverage the rich and readily-interpretable data from our study to demarcate distinct inflammatory states arising from each mutation. In all, our work provides a methodology for discovering novel regulators of CNS neuroimmune homeostasis and provides a validated analysis framework for identifying discrete neuroinflammatory modules that are engaged divergently across disease contexts.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M8934836GSM8934839GSM893483824247294978Mus musculus