{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE294nnn/GSE294996/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Mus musculus"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE294996"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Modular neuroinflammatory network discovery from large-scale phenotypic screening in genetically heterogeneous mice [part24]","description":"The central nervous system represents a uniquely immune-privileged environment, with inflammatory responses involving several resident CNS-specific cell types. While stereotyped cellular and transcriptional responses recur across varied diseases, relevant signaling pathways and regulatory networks are not fully understood. Here, we describe a screening platform based on high-throughput RNA-seq analysis and glial cell state gene set scores that enables multimodal investigation of inflammatory networks at scale. As proof-of-concept, we investigate genetically heterogeneous mice from a large-scale chemical mutagenesis screen to identify novel functionally relevant variants in six genes previously linked to human CNS disorders: Nrros, Ctsd, Smpd1, Idua, Nlrp1a, and Inpp5d . We leverage the rich and readily-interpretable data from our study to demarcate distinct inflammatory states arising from each mutation. In all, our work provides a methodology for discovering novel regulators of CNS neuroimmune homeostasis and provides a validated analysis framework for identifying discrete neuroinflammatory modules that are engaged divergently across disease contexts.","dates":{"publication":"2026/04/11"},"accession":"GSE294996","cross_references":{"GSM":["GSM8939814","GSM8939813","GSM8939812","GSM8939811","GSM8939818","GSM8939817","GSM8939816","GSM8939815","GSM8939819","GSM8939810","GSM8939825","GSM8939704","GSM8939703","GSM8939824","GSM8939702","GSM8939823","GSM8939822","GSM8939701","GSM8939708","GSM8939829","GSM8939828","GSM8939707","GSM8939706","GSM8939827","GSM8939705","GSM8939826","GSM8939709","GSM8939700","GSM8939821","GSM8939820","GSM8939803","GSM8939802","GSM8939801","GSM8939800","GSM8939807","GSM8939806","GSM8939805","GSM8939804","GSM8939809","GSM8939808","GSM8939692","GSM8939691","GSM8939690","GSM8939696","GSM8939695","GSM8939694","GSM8939693","GSM8939699","GSM8939698","GSM8939697","GSM8939679","GSM8939670","GSM8939791","GSM8939790","GSM8939674","GSM8939795","GSM8939673","GSM8939794","GSM8939672","GSM8939793","GSM8939671","GSM8939792","GSM8939799","GSM8939678","GSM8939677","GSM8939798","GSM8939676","GSM8939797","GSM8939796","GSM8939675","GSM8939681","GSM8939680","GSM8939685","GSM8939684","GSM8939683","GSM8939682","GSM8939689","GSM8939688","GSM8939687","GSM8939686","GSM8939659","GSM8939779","GSM8939658","GSM8939657","GSM8939778","GSM8939652","GSM8939773","GSM8939651","GSM8939772","GSM8939650","GSM8939771","GSM8939770","GSM8939656","GSM8939777","GSM8939776","GSM8939655","GSM8939654","GSM8939775","GSM8939653","GSM8939774","GSM8939669","GSM8939668","GSM8939789","GSM8939780","GSM8939663","GSM8939784","GSM8939662","GSM8939783","GSM8939782","GSM8939661","GSM8939660","GSM8939781","GSM8939788","GSM8939667","GSM8939666","GSM8939787","GSM8939665","GSM8939786","GSM8939785","GSM8939664","GSM8939759","GSM8939638","GSM8939637","GSM8939758","GSM8939636","GSM8939757","GSM8939756","GSM8939635","GSM8939639","GSM8939630","GSM8939751","GSM8939750","GSM8939634","GSM8939755","GSM8939633","GSM8939754","GSM8939753","GSM8939632","GSM8939631","GSM8939752","GSM8939649","GSM8939648","GSM8939769","GSM8939768","GSM8939647","GSM8939646","GSM8939767","GSM8939762","GSM8939641","GSM8939640","GSM8939761","GSM8939760","GSM8939645","GSM8939766","GSM8939765","GSM8939644","GSM8939643","GSM8939764","GSM8939642","GSM8939763","GSM8939616","GSM8939737","GSM8939736","GSM8939615","GSM8939614","GSM8939735","GSM8939613","GSM8939734","GSM8939619","GSM8939739","GSM8939618","GSM8939617","GSM8939738","GSM8939733","GSM8939612","GSM8939611","GSM8939732","GSM8939731","GSM8939730","GSM8939748","GSM8939627","GSM8939626","GSM8939747","GSM8939625","GSM8939746","GSM8939745","GSM8939624","GSM8939629","GSM8939628","GSM8939749","GSM8939740","GSM8939623","GSM8939744","GSM8939622","GSM8939743","GSM8939742","GSM8939621","GSM8939620","GSM8939741","GSM8939836","GSM8939715","GSM8939714","GSM8939835","GSM8939713","GSM8939834","GSM8939712","GSM8939833","GSM8939719","GSM8939839","GSM8939718","GSM8939717","GSM8939838","GSM8939716","GSM8939837","GSM8939711","GSM8939832","GSM8939831","GSM8939710","GSM8939830","GSM8939726","GSM8939725","GSM8939724","GSM8939723","GSM8939729","GSM8939728","GSM8939727","GSM8939722","GSM8939721","GSM8939720","GSM8939841","GSM8939840"],"GPL":["24247"],"GSE":["294996"],"taxon":["Mus musculus"]}}