GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE294nnn/GSE294996/primaryOK200TranscriptomicsMus musculusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE294996GEOGSEfalseModular neuroinflammatory network discovery from large-scale phenotypic screening in genetically heterogeneous mice [part24]The central nervous system represents a uniquely immune-privileged environment, with inflammatory responses involving several resident CNS-specific cell types. While stereotyped cellular and transcriptional responses recur across varied diseases, relevant signaling pathways and regulatory networks are not fully understood. Here, we describe a screening platform based on high-throughput RNA-seq analysis and glial cell state gene set scores that enables multimodal investigation of inflammatory networks at scale. As proof-of-concept, we investigate genetically heterogeneous mice from a large-scale chemical mutagenesis screen to identify novel functionally relevant variants in six genes previously linked to human CNS disorders: Nrros, Ctsd, Smpd1, Idua, Nlrp1a, and Inpp5d . We leverage the rich and readily-interpretable data from our study to demarcate distinct inflammatory states arising from each mutation. In all, our work provides a methodology for discovering novel regulators of CNS neuroimmune homeostasis and provides a validated analysis framework for identifying discrete neuroinflammatory modules that are engaged divergently across disease contexts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 musculus