{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE295nnn/GSE295340/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Genomics"],"species":["Mus musculus"],"gds_type":["Genome binding/occupancy profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE295340"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Polycomb Chromatin Topology Enables Long-Range Enhancer Recruitment during Craniofacial Development [ChIP-seq]","description":"Little is known about how three-dimensional chromatin topology shapes mammalian craniofacial development. In mouse cranial neural crest cells, a Polycomb Repressive Complex 2 (PRC2)-dependent chromatin architecture is established before migration. This configuration maintains craniofacial gene promoters poised and connects them with distal Polycomb tethering elements, positioning promoters in spatial proximity to future long-range enhancers. Deletion of Ezh2 disrupts this early topology, causing inappropriate gene derepression in post-migratory craniofacial subpopulations where these genes are normally silenced, and failure of long-range enhancer recruitment where activation is required, thereby impairing proper gene expression. We further identify a distal Polycomb tethering element essential for Hoxa2 enhancer recruitment across topologically associating domains. Thus, Polycomb acts not only as a transcriptional repressor, but also as a chromatin-folding organizer that prepares developmental genes for later activation, by facilitating subsequent recruitment of distal active enhancers previously not in contact. Polycomb-mediated topology therefore orchestrates the transition from progenitor plasticity to precise spatiotemporal control of morphogenetic gene programs during neural crest development and face formation.","dates":{"publication":"2026/04/08"},"accession":"GSE295340","cross_references":{"GSM":["GSM8945979","GSM8945978","GSM8945977","GSM8945976","GSM8945975","GSM8945974","GSM8945973","GSM8945972","GSM8945971","GSM8945970","GSM8945991","GSM8945990","GSM9562060","GSM9562061","GSM8945969","GSM8945968","GSM8945989","GSM8945967","GSM8945988","GSM8945966","GSM8945965","GSM8945987","GSM8945986","GSM8945964","GSM8945985","GSM8945963","GSM8945962","GSM8945984","GSM8945983","GSM8945982","GSM8945981","GSM8945980","GSM9562057","GSM9562056","GSM9562059","GSM9562058","GSM9562053","GSM9562052","GSM9562055","GSM9562054","GSM9562051","GSM9562050"],"GPL":["17021","24247"],"GSE":["295340"],"taxon":["Mus musculus"]}}