GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE295nnn/GSE295340/primaryOK200GenomicsMus musculusGenome binding/occupancy profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE295340GEOGSEfalsePolycomb Chromatin Topology Enables Long-Range Enhancer Recruitment during Craniofacial Development [ChIP-seq]Little is known about how three-dimensional chromatin topology shapes mammalian craniofacial development. In mouse cranial neural crest cells, a Polycomb Repressive Complex 2 (PRC2)-dependent chromatin architecture is established before migration. This configuration maintains craniofacial gene promoters poised and connects them with distal Polycomb tethering elements, positioning promoters in spatial proximity to future long-range enhancers. Deletion of Ezh2 disrupts this early topology, causing inappropriate gene derepression in post-migratory craniofacial subpopulations where these genes are normally silenced, and failure of long-range enhancer recruitment where activation is required, thereby impairing proper gene expression. We further identify a distal Polycomb tethering element essential for Hoxa2 enhancer recruitment across topologically associating domains. Thus, Polycomb acts not only as a transcriptional repressor, but also as a chromatin-folding organizer that prepares developmental genes for later activation, by facilitating subsequent recruitment of distal active enhancers previously not in contact. Polycomb-mediated topology therefore orchestrates the transition from progenitor plasticity to precise spatiotemporal control of morphogenetic gene programs during neural crest development and face formation.2026/04/08GSE295340GSM8945979GSM8945978GSM8945977GSM8945976GSM8945975GSM8945974GSM8945973GSM8945972GSM8945971GSM8945970GSM8945991GSM8945990GSM9562060GSM9562061GSM8945969GSM8945968GSM8945989GSM8945967GSM8945988GSM8945966GSM8945965GSM8945987GSM8945986GSM8945964GSM8945985GSM8945963GSM8945962GSM8945984GSM8945983GSM8945982GSM8945981GSM8945980GSM9562057GSM9562056GSM9562059GSM9562058GSM9562053GSM9562052GSM9562055GSM9562054GSM9562051GSM95620501702124247295340Mus musculus