{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE295nnn/GSE295473/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Other"],"species":["Mus musculus"],"gds_type":["Other"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE295473"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Polycomb Chromatin Topology Enables Long-Range Enhancer Recruitment during Craniofacial Development [PCHi-C]","description":"Little is known about how three-dimensional chromatin topology shapes mammalian craniofacial development. In mouse cranial neural crest cells, a Polycomb Repressive Complex 2 (PRC2)-dependent chromatin architecture is established before migration. This configuration maintains craniofacial gene promoters poised and connects them with distal Polycomb tethering elements, positioning promoters in spatial proximity to future long-range enhancers. Deletion of Ezh2 disrupts this early topology, causing inappropriate gene derepression in post-migratory craniofacial subpopulations where these genes are normally silenced, and failure of long-range enhancer recruitment where activation is required, thereby impairing proper gene expression. We further identify a distal Polycomb tethering element essential for Hoxa2 enhancer recruitment across topologically associating domains. Thus, Polycomb acts not only as a transcriptional repressor, but also as a chromatin-folding organizer that prepares developmental genes for later activation, by facilitating subsequent recruitment of distal active enhancers previously not in contact. Polycomb-mediated topology therefore orchestrates the transition from progenitor plasticity to precise spatiotemporal control of morphogenetic gene programs during neural crest development and face formation.","dates":{"publication":"2026/04/08"},"accession":"GSE295473","cross_references":{"GSM":["GSM8950959","GSM8950965","GSM8950954","GSM8950953","GSM8950964","GSM8950963","GSM8950952","GSM9557552","GSM8950951","GSM9557551","GSM8950962","GSM8950969","GSM8950958","GSM8950968","GSM8950957","GSM8950967","GSM8950956","GSM8950966","GSM8950955","GSM8950961","GSM8950950","GSM8950971","GSM8950960","GSM8950970"],"GPL":["19057"],"GSE":["295473"],"taxon":["Mus musculus"]}}