{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE295nnn/GSE295612/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Mus musculus"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE295612"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"APOE4 expression causes distinct effects on meningeal immunity, brain lipid composition, and cognition in male and female mice","description":"This experiment was designed to understand the distinct effects of sex and APOE genotype on the transcriptome of meningeal dural vascular and resident immune cells. After transcardiac perfusion with a buffered saline solution containing heparin and actinomycin D, meningeal dural whole mounts were immediately dissected from the skulls of 12-month-old male and female mice expressing APOE3 or APOE4 and digested into a single-cell suspension. After enrichment for CD31+ cells via positive selection using MACS, the cells were counted and approximately 5000 cells per sample were used to obtain single-cell gene expression libraries using 10x Genomics equipment and reagents, namely the Chromium Single Cell 3' Reagent Kits. The generated libraries were sequenced in 2 lanes of a NovaSeq 6000 S4 PE100 flow cell.","dates":{"publication":"2026/03/23"},"accession":"GSE295612","cross_references":{"GSM":["GSM8953716","GSM8953717","GSM8953718","GSM8953719","GSM8953712","GSM8953723","GSM8953724","GSM8953713","GSM8953725","GSM8953714","GSM8953715","GSM8953720","GSM8953721","GSM8953710","GSM8953722","GSM8953711"],"GPL":["24247"],"GSE":["295612"],"taxon":["Mus musculus"],"PMID":["[41895266]"]}}