GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE295nnn/GSE295679/primaryOK200TranscriptomicsSus scrofaExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE295679GEOGSEfalseTime-Resolved Transcriptomic Analysis of Mammary Ductal Development, Lactation Function Activation, and Glandular Involution in SowsTo investigate the dynamic changes in gene expression during mammary gland development and lactation in sows, mammary tissue samples were collected from 30 multiparous crossbred half-sibling sows (Danish Landrace × Yorkshire) housed under standardized conditions. Samples were obtained at five critical physiological stages: mid-gestation (Day 70), late gestation (Day 110), early lactation (Day 2 postpartum), peak lactation (Day 10 postpartum), and early involution (Day 2 post-weaning), with six biological replicates per stage. Parenchymal mammary tissue (0.5–1 cm³) was excised from a defined location below the third pair of teats and rapidly snap-frozen in liquid nitrogen for RNA preservation. All instruments were treated with RNase inhibitors to ensure RNA integrity. The collected samples were stored at –80°C for subsequent total RNA extraction and transcriptome sequencing, providing a valuable resource for elucidating molecular mechanisms underlying mammary gland remodeling and lactation regulation in sows.2026/05/20GSE295679GSM8955001GSM8954990GSM8955000GSM8954999GSM8955007GSM8954996GSM8954995GSM8955006GSM8955009GSM8954998GSM8955008GSM8954997GSM8954992GSM8955003GSM8955002GSM8954991GSM8955005GSM8954994GSM8955004GSM8954993GSM8955010GSM8955012GSM8955011GSM8955018GSM8955017GSM8955019GSM8955014GSM8955013GSM8955016GSM895501519176295679Sus scrofa[41265484]