{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE296nnn/GSE296154/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Other"],"species":["Mus musculus"],"gds_type":["Other"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE296154"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Sustained MYB activity drives emergent enhancer activation and precise enhancer-promoter interactions [Capture-C]","description":"Transcription factors (TFs) are key effectors of enhancer activity. MYB is a critical hematopoietic TF that is frequently dysregulated in cancer. Despite its well-established role, the exact mechanisms by which MYB influences enhancer function—and the specific stages of enhancer activation at which it operates—remain poorly understood. Using high resolution Micro-Capture-C, we show that upon MYB degradation, highly defined enhancer-promoter interactions at specific MYB binding sites are lost. Loss of these interactions, together with other hallmarks of enhancer activity—reduced H3 lysine-27 acetylation and enhancer RNA transcription—correlates with significant downregulation of target gene expression in leukemia, indicating that MYB mediates transcription activation via maintenance of enhancer function. When anchored to DNA within a gene desert region that is devoid of histone marks and active transcription, the MYB transactivation domain is sufficient and necessary for the nucleation of an enhancer-like region. This results in the activation of transcription from distal cryptic elements and the establishment of long-range chromatin interactions up to 400 kb away from the anchor point. Together, these results indicate that MYB activity alone is sufficient to induce long-range interactions and transcription, achieving this through highly precise enhancer-promoter crosstalk.","dates":{"publication":"2026/05/01"},"accession":"GSE296154","cross_references":{"GSM":["GSM8966039","GSM8966035","GSM8966036","GSM8966037","GSM8966038","GSM8966053","GSM8966031","GSM8966032","GSM8966033","GSM8966034","GSM8966050","GSM8966051","GSM8966030","GSM8966052","GSM8966028","GSM8966029","GSM8966024","GSM8966046","GSM8966047","GSM8966025","GSM8966048","GSM8966026","GSM8966027","GSM8966049","GSM8966042","GSM8966021","GSM8966043","GSM8966044","GSM8966022","GSM8966045","GSM8966023","GSM8966040","GSM8966041"],"GPL":["19057","16417"],"GSE":["296154"],"taxon":["Mus musculus"]}}