{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Csv":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE296nnn/GSE296187/suppl/GSE296187_gene_expression_matrix.csv.gz"],"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE296nnn/GSE296187/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Homo sapiens"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE296187"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Interleukin-23 promotes a pro-inflammatory Th17 cell state by stabilizing RORγt and suppressing glucocorticoid receptor activity I","description":"Interleukin-23 receptor (IL-23R) signaling is critical for the generation of pro-inflammatory CD4+ IL-17-producing T cells (Th17) that can drive autoimmune tissue inflammation, but the underlying mechanisms are not clear. We integrated phosphoproteomic and transcriptomic data downstream of IL-23R and IL-12R, which share a common subunit, to identify mechanisms engaged specifically by IL-23. We identified chromodomain helicase DNA-binding protein 1 (CHD1), an epigenetic regulator, and the glucocorticoid receptor (GR), a transcription factor (TF), as mediators of IL-23R signaling. IL-23R activation promoted CHD1 interaction with TF STAT3 and co-binding at the TF RORγt locus to enforce a pro-inflammatory Th17 state. Conversely, IL-23R signaling altered phosphorylation of the GR, thereby preventing its activation and nuclear translocation, ultimately impairing GR-driven inhibition of pro-inflammatory Th17 gene programs. Our findings uncover two mechanisms by which IL-23 promotes a pro-inflammatory Th17 cell state, offering potential therapeutic targets for treating Th17-driven autoimmune tissue inflammation and restoring homeostasis.","dates":{"publication":"2026/06/12"},"accession":"GSE296187","cross_references":{"GSM":["GSM8966619","GSM8966637","GSM8966615","GSM8966638","GSM8966616","GSM8966639","GSM8966617","GSM8966618","GSM8966633","GSM8966634","GSM8966635","GSM8966636","GSM8966630","GSM8966631","GSM8966632","GSM8966626","GSM8966627","GSM8966628","GSM8966629","GSM8966622","GSM8966623","GSM8966624","GSM8966625","GSM8966640","GSM8966641","GSM8966642","GSM8966620","GSM8966621"],"GPL":["16791"],"GSE":["296187"],"taxon":["Homo sapiens"]}}