GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE296nnn/GSE296277/primaryOK200OtherMus musculus Expression profiling by high throughput sequencingOtherhttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE296277GEOGSEfalseDistinct tissue niches contribute to prostate TRM cell differentiation and heterogeneity [scRNA-seq]The prostate is an important exocrine organ, a barrier tissue of the male reproductive system, and a common site of malignancy, yet prostate CD8+ T cells remain largely uncharacterized. Here, we show that a protective, heterogeneous pool of long-lived, tissue-resident memory CD8+ T (Trm) cells forms in the prostate following acute infection in mice. Characterization of prostate Trm cell differentiation over time and functional interrogation of cytokine TGFβ-, IL-7-, and IL-15 signaling revealed niche-dependent phenotypic and functional diversity arising from distinct prostate stromal and glandular epithelial niches in both mice and humans. For instance, the Trm-promoting cytokines IL-15 and TGFβ were highest in the prostate epithelium, where CD8+ T cells were most persistent, cytotoxic, and enriched for the Trm molecular program. In sum, we provide a spatial framework for prostate Trm cell differentiation, charting discrete tissue regions that influence T cell fate through dynamic regulation of localized signals.2026/03/31GSE296277GSM8967922GSM896792321103296277Mus musculus