{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE296nnn/GSE296292/"]},"type":"primary"},"statusCodeValue":200,"statusCode":"OK"}],"scores":null,"additional":{"omics_type":["Other"],"species":[" synthetic construct","Homo sapiens"],"gds_type":["Other"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE296292"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"A genome-wide functional analysis of conserved intronic regions reveals essential roles for speckle-associated detained introns [CHyMErA]","description":"Thousands of human introns harbour extended regions of high evolutionarily conservation that have not been previously characterized. A survey of these sequences reveals that they are associated with intron retention and enriched in genes that function in RNA processing, chromatin remodelling and neuronal biology. Using a dual CRISPR-Cas editing approach, we targeted 2,600 of these regions for deletion and observe that a subset of these perturbations affectaffects cell growth. Many of these ‘fitness’ sequences affect intron retention and or expression levels of their host genes. Deletions in nuclear speckle-associated retained introns in the FNBP4 and DDX5 genes are further linked to downstream effects on cell growth-related genes and intron retention, respectively. The DDX5 deletion additionally results in the accumulation of R-loops overlapping first introns in speckle-proximal genes. Overall, the results highlight multifactetedmultifaceted, critical roles of highly conserved intronic sequences in the control of gene regulation, R-loop resolution and cell growth.","dates":{"publication":"2026/06/18"},"accession":"GSE296292","cross_references":{"GSM":["GSM8968159","GSM8968158","GSM8968177","GSM8968155","GSM8968154","GSM8968176","GSM8968157","GSM8968179","GSM8968178","GSM8968156","GSM8968151","GSM8968173","GSM8968172","GSM8968150","GSM8968175","GSM8968153","GSM8968174","GSM8968152","GSM8968171","GSM8968170","GSM8968148","GSM8968169","GSM8968147","GSM8968149","GSM8968166","GSM8968144","GSM8968143","GSM8968165","GSM8968146","GSM8968168","GSM8968167","GSM8968145","GSM8968162","GSM8968161","GSM8968164","GSM8968163","GSM8968180","GSM8968160","GSM8968182","GSM8968181"],"GPL":["24676","26526"],"GSE":["296292"],"taxon":[" synthetic construct","Homo sapiens"]}}