{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE296nnn/GSE296306/"]},"type":"primary"},"statusCodeValue":200,"statusCode":"OK"}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Homo sapiens"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE296306"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"A genome-wide functional analysis of conserved intronic regions reveals essential roles for speckle-associated detained introns [RNAseq_candidates.RPE1]","description":"Thousands of human introns harbour extended regions of high evolutionarily conservation that have not been previously characterized. A survey of these sequences reveals that they are associated with intron retention and enriched in genes that function in RNA processing, chromatin remodelling and neuronal biology. Using a dual CRISPR-Cas editing approach, we targeted 2,600 of these regions for deletion and observe that a subset of these perturbations affectaffects cell growth. Many of these ‘fitness’ sequences affect intron retention and or expression levels of their host genes. Deletions in nuclear speckle-associated retained introns in the FNBP4 and DDX5 genes are further linked to downstream effects on cell growth-related genes and intron retention, respectively. The DDX5 deletion additionally results in the accumulation of R-loops overlapping first introns in speckle-proximal genes. Overall, the results highlight multifactetedmultifaceted, critical roles of highly conserved intronic sequences in the control of gene regulation, R-loop resolution and cell growth.","dates":{"publication":"2026/06/18"},"accession":"GSE296306","cross_references":{"GSM":["GSM8968415","GSM8968412","GSM8968411","GSM8968414","GSM8968413","GSM8968397","GSM8968396","GSM8968410","GSM8968399","GSM8968398","GSM8968393","GSM8968392","GSM8968395","GSM8968394","GSM8968391","GSM8968390","GSM8968409","GSM8968408","GSM8968405","GSM8968404","GSM8968407","GSM8968406","GSM8968401","GSM8968400","GSM8968389","GSM8968403","GSM8968402","GSM8968386","GSM8968385","GSM8968388","GSM8968387","GSM8968382","GSM8968384","GSM8968383"],"GPL":["24676"],"GSE":["296306"],"taxon":["Homo sapiens"]}}