GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE296nnn/GSE296372/ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE296nnn/GSE296372/suppl/GSE296372_Rat_transgen_multiomeRNA_refined_all_SeuratObj_N5.h5Seuratftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE296nnn/GSE296372/suppl/GSE296372_Rat_Transgen_NAc_scATAC_clusterRat_ArchRProject.tar.gzprimary200OKGenomicsRattus norvegicusGenome binding/occupancy profiling by high throughput sequencing Expression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE296372GEOGSEfalsePaternal methamphetamine exposure enhances methamphetamine susceptibility in male rat offspringWe previously demonstrated that male progeny of cocaine-experienced sires resisted cocaine, expressed as delayed acquisition of cocaine self-administration. Here, we sought to determine whether this phenotype extended to another psychostimulant, methamphetamine. Surprisingly, relative to saline-sired rats, male, but not female, methamphetamine-sired offspring demonstrated increased susceptibility to methamphetamine taking. Single nuclei RNA- and ATAC-sequencing of the nucleus accumbens was used to identify molecular drivers of phenotypes in cocaine- and methamphetamine-sired offspring. Sequencing results revealed unique profiles of gene expression and open chromatin in offspring nucleus accumbens. Differential gene expression in cocaine-resistant, cocaine-sired offspring and methamphetamine-susceptible, methamphetamine-sired offspring illuminates potential novel treatment targets for psychostimulant use disorders.2026/08/12GSE296372GSM8969354GSM8969353GSM8969356GSM8969355GSM8969350GSM8969352GSM8969351GSM8969347GSM8969358GSM8969357GSM8969349GSM896934825947296372Rattus norvegicus