{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE296nnn/GSE296378/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Mus musculus"],"gds_type":[" Other","Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE296378"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Regulatory Effects of Smoking Cessation on the Cellular Microenvironment and Differentially Expressed Genes in Precancerous Lesions of Pulmonary Nodules in Mice based on Single-Cell RNA-Sequencing and Immune Repertoire-Sequencing","description":"Smoking cessation is known to reduce lung cancer progression, but its impact on precancerous lesions remains largely unexplored. In this study, a smoking-induced mouse model of pulmonary precancerous lesions was established using 6-week benzo[a]pyrene (B[a]P) exposure. Lung nodules were identified through micro-MRI imaging and histopathological evaluation by H&E staining and fluorescence microscopy. Smoking cessation interventions were applied for 4 and 8 weeks following lesion confirmation. Nodule size was quantified with Generic Medical Imaging software. Single-cell RNA sequencing (scRNA-seq) and immune repertoire sequencing (IR-seq) were performed to analyze immune cell composition, intercellular communication pathways, and receptor diversity. These approaches provide a comprehensive framework for investigating the cellular and molecular changes associated with smoking cessation in early lung lesion development.","dates":{"publication":"2026/03/29"},"accession":"GSE296378","cross_references":{"GSM":["GSM8969411","GSM8969410","GSM8969409","GSM8969406","GSM8969405","GSM8969408","GSM8969407","GSM8969413","GSM8969402","GSM8969412","GSM8969404","GSM8969403"],"GPL":["24247"],"GSE":["296378"],"taxon":["Mus musculus"]}}