GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE296nnn/GSE296378/primaryOK200TranscriptomicsMus musculus OtherExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE296378GEOGSEfalseRegulatory Effects of Smoking Cessation on the Cellular Microenvironment and Differentially Expressed Genes in Precancerous Lesions of Pulmonary Nodules in Mice based on Single-Cell RNA-Sequencing and Immune Repertoire-SequencingSmoking cessation is known to reduce lung cancer progression, but its impact on precancerous lesions remains largely unexplored. In this study, a smoking-induced mouse model of pulmonary precancerous lesions was established using 6-week benzo[a]pyrene (B[a]P) exposure. Lung nodules were identified through micro-MRI imaging and histopathological evaluation by H&E staining and fluorescence microscopy. Smoking cessation interventions were applied for 4 and 8 weeks following lesion confirmation. Nodule size was quantified with Generic Medical Imaging software. Single-cell RNA sequencing (scRNA-seq) and immune repertoire sequencing (IR-seq) were performed to analyze immune cell composition, intercellular communication pathways, and receptor diversity. These approaches provide a comprehensive framework for investigating the cellular and molecular changes associated with smoking cessation in early lung lesion development.2026/03/29GSE296378GSM8969411GSM8969410GSM8969409GSM8969406GSM8969405GSM8969408GSM8969407GSM8969413GSM8969402GSM8969412GSM8969404GSM896940324247296378Mus musculus