{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Csv":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE296nnn/GSE296482/suppl/GSE296482_pTh17_in_vitro_2d_GC_TGF.csv.gz","ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE296nnn/GSE296482/suppl/GSE296482_Cyp11a1_KO_WT_CNS.csv.gz","ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE296nnn/GSE296482/suppl/GSE296482_2D2_npTh17_GR.csv.gz"],"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE296nnn/GSE296482/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Mus musculus"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE296482"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"A cell-intrinsic glucocorticoid biosynthesis and sensing circuit maintains homeostatic Th17 cell state [RNA-seq]","description":"T helper 17 (Th17) cells are heterogeneous; homeostatic Th17 (Th17Hom) cells maintain tissue homeostasis and pro-inflammatory Th17 (Th17Inf) cells drive autoimmune inflammation. IL-23 drives Th17Inf, but the signals that maintain Th17Hom remain unclear. Here, we found that differential glucocorticoid (GC) production downstream of CYP11A1, a critical enzyme for steroidogenesis, distinguished Th17Hom cells from Th17Inf cells. Although TCR signaling opened the Cyp11a1 locus, TGFβ1 and IL-6, key cytokines for Th17Hom cell differentiation, maintained and amplified Cyp11a1. Th17Hom cell-derived GC signaled through the glucocorticoid receptor (GR), which was higher in Th17Hom cells compared to Th17Inf cells, thereby forming a circuit that maintained the homeostatic state. Integration of multi-omics data from CYP11A1- and GR-deficient Th17Hom cells revealed a gene network underlying this circuit. TGFβ1, a key node in the network, restored GC sensing to Th17Inf. We thus identify a GC signaling circuit that maintains Th17 homeostasis with implications for treating Th17-mediated autoimmunity.","dates":{"publication":"2026/06/12"},"accession":"GSE296482","cross_references":{"GSM":["GSM8971090","GSM8971091","GSM8971092","GSM8971093","GSM8971094","GSM8971095","GSM8971096","GSM8971097","GSM8971075","GSM8971098","GSM8971076","GSM8971077","GSM8971099","GSM8971078","GSM8971079","GSM8971080","GSM8971081","GSM8971082","GSM8971083","GSM8971103","GSM8971104","GSM8971105","GSM8971084","GSM8971085","GSM8971086","GSM8971087","GSM8971088","GSM8971100","GSM8971089","GSM8971101","GSM8971102"],"GPL":["19057","21626"],"GSE":["296482"],"taxon":["Mus musculus"]}}