GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE296nnn/GSE296587/primaryOK200GenomicsMus musculusGenome binding/occupancy profiling by high throughput sequencing Otherhttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE296587GEOGSEfalseSequencing DNA methylation and hydroxymethylation at co-occurring chromatin featuresEpigenetic modifications govern chromatin dynamics and cell state. However, current methods cannot simultaneously resolve the presence of multiple DNA modifications at co-occurring chromatin-associated features. It is thus not clear how these features are physically coupled and how their combinations regulate genome function. To address this key question, we report 6-base-CUT&Tag, a method for simultaneous 6-base sequencing at target chromatin features. Using 6-base-CUT&Tag to profile 5mC and 5hmC at co-occurring histone modifications in mouse embryonic stem cells, we identify histone mark-specific signatures of methylation and hydroxymethylation to improve discrimination of different functional enhancer states.2026/01/15GSE296587GSM9349170GSM9349171GSM9349174GSM9349175GSM9349172GSM9349173GSM8972984GSM8972985GSM8972986GSM8972987GSM8972988GSM9349178GSM9349179GSM9349176GSM9349177GSM8972980GSM8972981GSM8972982GSM8972983GSM9349163GSM9349164GSM9349161GSM9349162GSM8972977GSM8972978GSM8972979GSM9349167GSM9349168GSM9349165GSM9349166GSM93491693017224247296587Mus musculus[41667493]