GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE296nnn/GSE296721/primaryOK200Transcriptomicssynthetic RNAExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE296721GEOGSEfalseConformational Dynamics in RNA Cleavage Specificity During DicingIn this study, using massively parallel dicing assays and cryo-electron microscopy, we identify a previously unrecognized guanosine-favored (G-favored) binding pocket in DICER that exhibits a striking specificity for guanosine. This discovery fundamentally redefines the canonical view of DICER substrate recognition, as it contrasts with the known uridine-favored (U-favored) pocket, which binds uridine, adenine, and cytosine while excluding guanosine. We demonstrate that these distinct G- and U-favored pockets govern the generation of 21-nt and 22-nt small RNAs, respectively, revealing a new layer of control in small RNA biogenesis2026/05/20GSE296721GSM8975290GSM8975292GSM8975291GSM8975294GSM8975293GSM8975296GSM8975295GSM8975298GSM8975297GSM8975311GSM8975310GSM8975299GSM8975306GSM8975305GSM8975308GSM8975307GSM8975309GSM8975287GSM8975286GSM8975289GSM8975300GSM8975288GSM8975302GSM8975301GSM8975304GSM897530333226296721synthetic RNA[41781616]