GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE296nnn/GSE296897/primaryOK200OtherMus musculusOtherhttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE296897GEOGSEfalseHigh-resolution spatial transcriptomics of DARLIN mouse [CRISPR array amplicon]Spatially-resolved single-cell lineage tracing is a powerful approach for studying cell lineages and fate decisions in situ. However, existing studies are limited to specific cell types such as lymphocytes and cancer cells. We recently developed DARLIN, a lineage tracing mouse model that has massive lineage barcodes and efficient barcode recovery. Here, we present Spatio-DARLIN, a generic spatial lineage tracing tool that integrates sequencing-based spatial transcriptomics with DALRIN mouse. Applying it to the small intestine, we observed a high-quality transcriptome with nearly single-cell resolution and recovered ~25% cells with reliable lineage barcodes, resulting in ~7000 clones with >1 cell per tissue section. These clones showed highly reproducible spatial localization across adjacent tissue sections, confirming their accuracy. With this high-throughput approach, we observed that intestinal epithelial clones were localized along the crypt-villus axis and underwent vertical expansion over a longer tracing period. Since DARLIN is publicly available and labels cells across most tissues, Spatio-DARLIN offers an accessible and efficient approach for spatial lineage tracing across various mouse tissues with high resolution.2026/04/12GSE296897GSM8979593GSM8979592GSM8979591GSM8979590GSM8979595GSM8979594GSM9463750GSM9463752GSM9463751GSM9463754GSM9463753GSM9463756GSM9463755GSM9206540GSM9206541GSM9206542GSM9475364GSM9475363GSM9475362GSM9475361GSM9475360GSM9463749GSM8979575GSM8979574GSM8979573GSM8979572GSM9475353GSM9475352GSM9475351GSM9475350GSM8979579GSM8979578GSM9463757GSM9475359GSM8979577GSM9475358GSM8979576GSM9475357GSM9475356GSM9475355GSM9475354GSM8979582GSM8979581GSM8979580GSM8979586GSM8979585GSM8979584GSM8979583GSM8979589GSM9475349GSM8979588GSM9475348GSM8979587GSM9475347GSM9475346GSM947534524247296897Mus musculus