GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE297nnn/GSE297089/primaryOK200GenomicsArabidopsis thalianaGenome binding/occupancy profiling by high throughput sequencing Expression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE297089GEOGSEfalseThe lncRNA BAMBOO decoys NF-YA2 transcription factor from the promoter of its targets in Arabidopsis lateral rootsPlant developmental plasticity emerges from transcriptional reprogramming in response to environmental shifts, which largely depends on the activity of transcription factors (TFs). In Arabidopsis thaliana, NF-YA2 and NF-YA10 (Nuclear Factor A2 and A10) are TFs post-transcriptionally regulated by microRNAs, participating in a regulatory hub controlling root architecture. Here, RNA-seq and ChIP-seq approaches served to identify NF-YA2 direct target genes involved in lateral root (LR) development, water deprivation and hormone pathways. Furthermore, we searched for NFYA2 potential RNA partners by using RIP-seq and identified a long noncoding RNA (lncRNA) named BAMBOO after its counterpart PANDA, which interacts with mammalian NF-YA. BAMBOO is dynamically regulated during LR development and bamboo mutant plants exhibit a NF-YA2-related root developmental phenotype. bamboo plants share half of its deregulated genes with the NF-YA2 regulome. Furthermore, overexpressing the lncRNA BAMBOO disturbed NF-YA2 binding to its target promoters suggesting an RNA-based competition of target recognition by NF-YA2 in chromatin. Hence, the action of lncRNAs at the organismal level underscores their capacity to intricately modulate TF activity throughout targeted developmental processes.2026/05/13GSE297089GSM8983510GSM8983521GSM8983520GSM8983518GSM8983517GSM8983516GSM8983515GSM8983525GSM8983514GSM8983513GSM8983524GSM8983523GSM8983512GSM8983522GSM8983511GSM8983509GSM898351919580297089Arabidopsis thaliana