GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE297nnn/GSE297180/primaryOK200TranscriptomicsHomo sapiensExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE297180GEOGSEfalseMagnetic Field Suppresses Non-Small Cell Lung Cancer via RSRP1-Mediated Secretory Signaling AxisMagnetic field therapy holds promise for tumor treatment, yet its underlying mechanisms remain controversial. The traditional ROS theory fails to fully explain the observed magnetic field-induced inhibition. Building on prior findings, we identified the key target of magnetic field-mediated tumor suppression and established a magnetic inhibition model. Integrative multi-omics analysis (transcriptome + proteome) of 293T and A549 cells exposed to (5 mT, 20 Hz) magnetic fields pinpointed RSRP1 as a critical molecular target. We experimentally validated RSRP1's essential role using CRISPR-Cas9-mediated gene disruption and TET-on inducible expression systems. Proteomic analysis revealed that magnetic field exposure (5 mT, 20 Hz) induced >200-fold upregulation of RSRP1 protein in both non-tumorigenic 293T and tumorigenic A549 cells. CRISPR-Cas9-mediated RSRP1 knockout completely abolished A549 cells' responsiveness to magnetic fields. Notably, restoring RSRP1 expression via a doxycycline-inducible system reinstated magnetic field-induced secretion of inhibitory factors, with conditioned media from exposed cells significantly inhibiting naive tumor cell proliferation (p<0.05). Magnetic field (5 mT, 20 Hz) exposure transcriptionally upregulated RSRP1 endogenously, as confirmed by transcriptomic and qPCR analyses, while exogenously overexpressed RSRP1 via a doxycycline-inducible system remained unresponsive, indicating that magnetic regulation requires endogenous transcriptional control. This study establishes a "magnetic field→RSRP1→secretory factor→target response" cascade, providing a transformative framework for non-invasive tumor therapy.2026/05/12GSE297180GSM8985070GSM8985071GSM8985072GSM898507329480297180Homo sapiens