{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE297nnn/GSE297816/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Mus musculus"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE297816"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Early-life RNA sequencing of colon stromal cells and iNKT cells","description":"The early-life intestinal microenvironment plays a pivotal role in shaping immune cell development, yet the mechanisms regulating colonic invariant natural killer T (iNKT) cells remain incompletely understood. To investigate how embryonic macrophages regulate iNKT cells during early life, we collected Day 9 whole-colon lamina propria cells after embryonic macrophage depletion and performed both bulk RNA sequencing and single-cell RNA sequencing. We also performed bulk RNA sequencing on distinct colonic stromal cell populations, conventional αβT cells, and iNKT cells under homeostatic early-life conditions to identify factors that directly imprint iNKT cells during this developmental period.","dates":{"publication":"2026/04/07"},"accession":"GSE297816","cross_references":{"GSM":["GSM9000219","GSM9000218","GSM9000217","GSM9000205","GSM9000227","GSM9000226","GSM9000204","GSM9000225","GSM9000203","GSM9000202","GSM9000224","GSM9000223","GSM9000201","GSM9000222","GSM9000189","GSM9000200","GSM9000188","GSM9000221","GSM9000220","GSM9000209","GSM9000208","GSM9000207","GSM9000206","GSM9000216","GSM9000215","GSM9000214","GSM9000213","GSM9000212","GSM9000211","GSM9000199","GSM9000210","GSM9000198","GSM9000197","GSM9000196","GSM9000195","GSM9000194","GSM9000193","GSM9000192","GSM9000191","GSM9000190"],"GPL":["19057","21273","24247"],"GSE":["297816"],"taxon":["Mus musculus"]}}