GEOTranscriptomicsMus musculusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE297816GEOGSEfalseEarly-life RNA sequencing of colon stromal cells and iNKT cellsThe early-life intestinal microenvironment plays a pivotal role in shaping immune cell development, yet the mechanisms regulating colonic invariant natural killer T (iNKT) cells remain incompletely understood. To investigate how embryonic macrophages regulate iNKT cells during early life, we collected Day 9 whole-colon lamina propria cells after embryonic macrophage depletion and performed both bulk RNA sequencing and single-cell RNA sequencing. We also performed bulk RNA sequencing on distinct colonic stromal cell populations, conventional αβT cells, and iNKT cells under homeostatic early-life conditions to identify factors that directly imprint iNKT cells during this developmental period.2026/04/07GSE297816GSM9000219GSM9000218GSM9000217GSM9000205GSM9000227GSM9000226GSM9000204GSM9000225GSM9000203GSM9000202GSM9000224GSM9000223GSM9000201GSM9000222GSM9000200GSM9000189GSM9000188GSM9000221GSM9000220GSM9000209GSM9000208GSM9000207GSM9000206GSM9000216GSM9000215GSM9000214GSM9000213GSM9000212GSM9000211GSM9000199GSM9000210GSM9000198GSM9000197GSM9000196GSM9000195GSM9000194GSM9000193GSM9000192GSM9000191GSM9000190190572127324247297816Mus musculus