GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE297nnn/GSE297828/primaryOK200TranscriptomicsMus musculusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE297828GEOGSEfalseOxygen-Tolerant Hydrogenase-Ag Nanoclusters-Peptide Hydrogel for Light-Driven H₂ Therapy in Diabetic Wound HealingDiabetic wound healing is hindered by excessive reactive oxygen species (ROS), while conventional hydrogen (H₂) delivery methods lack localized efficacy. Escherichia coli [NiFe]-hydrogenase 2 (Hyd-2), though promising for H₂ production, suffers from oxygen sensitivity. Here, we engineer a peptide hydrogel (Fmoc-KYF-AgNCs) that shields Hyd-2 from oxygen inactivation, enabling sustained light-driven H₂ generation under aerobic conditions (retaining 80% activity after 12 hours). In vitro, the hydrogel scavenges ROS in LPS-stimulated macrophages, suppresses pro-inflammatory cytokines, and enhances cell survival. In diabetic mice, the hydrogel accelerates wound closure (87% by day 11), reduces oxidative stress, promotes angiogenesis, and reprograms macrophages toward an anti-inflammatory M2 phenotype. Transcriptomic profiling reveals downregulation of inflammatory pathways and upregulation of tissue repair genes. This oxygen-tolerant system bridges enzymatic catalysis with precision medicine, offering a translatable platform for diabetic wound therapy and expanding hydrogenase applications in oxidative stress-related disorders.2026/05/21GSE297828GSM9000449GSM9000459GSM9000469GSM9000458GSM9000468GSM9000457GSM9000467GSM9000456GSM9000466GSM9000455GSM9000454GSM9000465GSM9000464GSM9000453GSM9000463GSM9000452GSM9000451GSM9000462GSM9000472GSM9000461GSM9000450GSM9000471GSM9000460GSM900047028330297828Mus musculus