GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE297nnn/GSE297902/primaryOK200TranscriptomicsMus musculusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE297902GEOGSEfalseTBK1 enhances antifungal immunity by forming a SRC-TBK1 positive feedback loop in phagosomes of macrophagesTBK1 is a serine/threonine kinase known for its role in antiviral immunity. Here, we identify TBK1 as a key amplifier of antifungal immune responses. Using a mouse model of Candida albicans infection, we show that TBK1 is recruited to phagosomes via SRC-SHP2 signaling and phosphorylates SRC to establish a positive feedback loop. This enhances downstream cytokine production, including IL-6 and TNF-α, and promotes fungal clearance. Bone marrow-derived macrophages (BMDMs) from TBK1fl/fl and TBK1fl/fl Lyz2-Cre mice were stimulated with various fungal components, and RNA-seq was performed to assess transcriptional changes. Our findings define a new role for TBK1 in antifungal host defense and suggest TBK1 as a potential therapeutic target.2025/06/14GSE297902GSM9001690GSM9001680GSM9001681GSM9001670GSM9001671GSM9001682GSM9001683GSM9001672GSM9001684GSM9001673GSM9001685GSM9001674GSM9001675GSM9001686GSM9001687GSM9001676GSM9001688GSM9001677GSM9001678GSM9001689GSM9001679GSM900166932159297902Mus musculus[40638388]