{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE298nnn/GSE298103/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Mus musculus"],"gds_type":["Expression profiling by array"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE298103"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Microarray analysis of hearts with TGFb deficient cardiomyocytes.","description":"Transforming growth factor β (TGFβ) is a secreted growth factor that, in early development, is produced by cardiac myocytes and impacts fibroblasts, driving differentiation and into a differentiated state to synthesize ECM and matricellular proteins. These, in turn, impact cardiomyocyte maturation through cell-cell interactions, ECM-cellular interactions, and paracrine factors. Deletion of Tgfb1, Tgfb2, Tgfb3 genes (TGFβ ligands) from cardiomyocytes during early development in a genetically modified mouse line was found to result in cardiac dysfunction by 6 weeks of age. This indicated a definitive role for TGFβ ligands produced by cardiac myocytes in cardiac development and function. The goal of this microarray project was to transcriptionally profile hearts with cardiomyocytes that were deficient in TGFβ ligand production. In order to transcriptionally profile hearts encompassing cardiomyocytes with deficient TGFb signaling, genetically-modified mouse lines were bred in which genes encoding the TGFb ligands could be knocked out specifically in cardiomyocytes in a Cre recombinase-dependent manner. This was achieved utilizing the aMHC-Cre transgenic line where Cre-recombinase is expressed exclusively in cardiomyocytes under the aMHC promoter [PMID: 9202069]. Whole hearts were harvested from 6-week old control [aMHC-Cre transgenic] and mutant [double knockouts (DKO) with TGFb 2 and 3 deleted and triple knockouts (TKO) with TGFb 1, 2 and 3 deleted] mice. Microarray analysis was performed on total RNA isolated from biological replicates of these hearts at the Gene Expression Core Facility (CCHMC) using the Affymetrix Cariom S platform. Differential gene expression was determined by bioinformatic analysis (CEL files) using Transcriptome Analysis Console 9.","dates":{"publication":"2026/09/10"},"accession":"GSE298103","cross_references":{"GSM":["GSM9008364","GSM9008363","GSM9008359","GSM9008362","GSM9008361","GSM9008360"],"GPL":["23038"],"GSE":["298103"],"taxon":["Mus musculus"]}}