GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE298nnn/GSE298436/primaryOK200TranscriptomicsMus musculusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE298436GEOGSEfalseNobiletin targets CHEK1-H3K27ac-CENPM axis and suppresses the malignancy of colorectal cancerPharmacological intervention targeting super-enhancer activity represents a promising therapeutic approach to suppress the expression of oncogenes. The challenges remain in unveiling the key factors accounting for the hyperactive super-enhancers in cancers and small molecules for the functional inhibition. In this study, we identified CHEK1 as a critical factor for sustaining the high level H3K27ac enrichment in the super-enhancer regions of oncogenes. Nobiletin was found as an inhibitor of CHEK1. We made these findings in the efforts to decipher the molecular mechanism underlying a modified Traditional Chinese Medicine formula Liujunzi Decoctiom (mLJZD). We experimentally confirmed the anti-cancer function of mLJZD both in vitro and in vivo. Through an interactive analysis of RNA profiling data, we identified oncogenic CENPM as a critical effector gene that is downregulated by mLJZD2026/05/31GSE298436GSM9014259GSM9014258GSM9014265GSM9014264GSM9014263GSM9014262GSM9014261GSM901426024247298436Mus musculus