{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE298nnn/GSE298512/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Homo sapiens"],"gds_type":[" Other","Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE298512"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Single Cell Glycome and Transcriptome Profiling Enabled by a Library of Anti-glycan Antibodies","description":"Despite their importance in cellular processes and as clinical targets, glycans remain difficult to study with existing technologies. A major barrier to developing tools to better study glycans is the dearth of high-quality, well-characterized anti-glycan antibodies. To create a resource for optimally selecting specific anti-glycan antibodies and understanding their general properties, we generated a publicly accessible database of 650 anti-glycan antibody sequences, recombinantly expressed a library of 154 of these antibodies, and characterized their binding properties using a glycan microarray. We DNA-barcoded a subset of these antibodies to create a platform enabling simultaneous high-throughput glycome and transcriptome profiling at the single-cell level. Using this platform, we profiled the two isogenic colorectal cancer cell lines, SW480 and SW620. The results reveal a variety of glycans associated with cancer stem cells and metastasis and demonstrate the power of integrating the glycome into multi-omic efforts to discover biomarkers and therapeutic targets.","dates":{"publication":"2026/05/20"},"accession":"GSE298512","cross_references":{"GSM":["GSM9015936","GSM9015937","GSM9015938","GSM9015939","GSM9015940","GSM9015941","GSM9015934","GSM9015935"],"GPL":["18573","24676"],"GSE":["298512"],"taxon":["Homo sapiens"],"PMID":["[41380686]"]}}