{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE298nnn/GSE298800/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Other"],"species":["Saccharomyces cerevisiae"],"gds_type":["Other"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE298800"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Mapping the latent CRBN-molecular glue degrader interactome","description":"Molecular glue degraders (MGDs) are a transformative modality in drug discovery. MGDs that work in concert with the E3 ligase CRL4CRBN can degrade a wide range of substrates with the help of specially tailored MGDs. To explore CRL4CRBN reprogrammability, we tested whether reported CRBN-MGD substrates are part of a network of latent CRBN interactors, detectable already with generic CRBN-MGDs. Leveraging highly parallel interaction measurements (GluePCA) between CRL4CRBN and the family of human zinc-fingers (ZFs), we identified ~210 ZFs pre-bound to CRBN-pomalidomide, with the top binders reported as degradable through specific MGDs. To map latent CRBN-MGDs interactions proteome-wide, and thus define the immediately accessible CRBN target space, we combined AI-derived protein surface queries (MaSIF-mimicry) with GluePCA. This pipeline allowed identifying 43 novel CRBN-pomalidomide binders, thereby providing privileged starting points for MGD development. We expect this binding-focused, highly parallel, workflow to be readily applicable to other MGD/E3 ligase systems.","dates":{"publication":"2026/06/08"},"accession":"GSE298800","cross_references":{"GSM":["GSM9023919","GSM9023916","GSM9023915","GSM9023918","GSM9023917","GSM9023912","GSM9023911","GSM9023914","GSM9023913","GSM9234574","GSM9023921","GSM9234573","GSM9023920","GSM9234579","GSM9234576","GSM9234575","GSM9234578","GSM9234577","GSM9023909","GSM9023908","GSM9023927","GSM9023905","GSM9023904","GSM9023926","GSM9023907","GSM9023929","GSM9023928","GSM9023906","GSM9023923","GSM9023901","GSM9023922","GSM9023925","GSM9023903","GSM9023924","GSM9023902","GSM9023930","GSM9234583","GSM9234582","GSM9023910","GSM9234584","GSM9234581","GSM9234580"],"GPL":["35358","17342","19756"],"GSE":["298800"],"taxon":["Saccharomyces cerevisiae"]}}