GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE298nnn/GSE298800/primaryOK200OtherSaccharomyces cerevisiaeOtherhttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE298800GEOGSEfalseMapping the latent CRBN-molecular glue degrader interactomeMolecular glue degraders (MGDs) are a transformative modality in drug discovery. MGDs that work in concert with the E3 ligase CRL4CRBN can degrade a wide range of substrates with the help of specially tailored MGDs. To explore CRL4CRBN reprogrammability, we tested whether reported CRBN-MGD substrates are part of a network of latent CRBN interactors, detectable already with generic CRBN-MGDs. Leveraging highly parallel interaction measurements (GluePCA) between CRL4CRBN and the family of human zinc-fingers (ZFs), we identified ~210 ZFs pre-bound to CRBN-pomalidomide, with the top binders reported as degradable through specific MGDs. To map latent CRBN-MGDs interactions proteome-wide, and thus define the immediately accessible CRBN target space, we combined AI-derived protein surface queries (MaSIF-mimicry) with GluePCA. This pipeline allowed identifying 43 novel CRBN-pomalidomide binders, thereby providing privileged starting points for MGD development. We expect this binding-focused, highly parallel, workflow to be readily applicable to other MGD/E3 ligase systems.2026/06/08GSE298800GSM9023919GSM9023916GSM9023915GSM9023918GSM9023917GSM9023912GSM9023911GSM9023914GSM9023913GSM9234574GSM9023921GSM9234573GSM9023920GSM9234579GSM9234576GSM9234575GSM9234578GSM9234577GSM9023909GSM9023908GSM9023927GSM9023905GSM9023904GSM9023926GSM9023907GSM9023929GSM9023928GSM9023906GSM9023923GSM9023901GSM9023922GSM9023925GSM9023903GSM9023924GSM9023902GSM9023930GSM9234583GSM9234582GSM9023910GSM9234584GSM9234581GSM9234580353581734219756298800Saccharomyces cerevisiae