{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE299nnn/GSE299271/"]},"type":"primary"},"statusCodeValue":200,"statusCode":"OK"}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Dichotomyctere nigroviridis"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE299271"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"New insights into SREB orphan receptor roles in the ovary, using comparative transcriptomics across 3 fishes","description":"Green spotted pufferfish ovary in vitro assays were completed using methods modified from Rajeswari et al., 2020. Briefly, female D. nigroviridis (n = 14) were euthanized in 300 mg/L buffered MS-222 (Syncaine®, tricaine methanesulfonate, Syndel, Ferndale, WA, USA), weighed, and measured (total length (mm), TL). Green spotted pufferfish ovaries from each fish were partitioned into 5 sections of approximately equal size. Each of four of these sections were immersed into an assigned well within a 24-well plate containing 990 μl of culture media (90% Leibovitz’s L-15 medium [Sigma Cat# L1518], 1% Penicillin-Streptomycin [Gibco™ Cat# 15070-063], 0.5% bovine serum albumin [Sigma Cat# 81053N]), with either a DMSO control or one of 3 potential agonist treatments, 8535 (5 μM), PT-91 (5 μM), or PNX-20 (100 nM). Agonists were dissolved in DMSO and added to their respective wells in 10μl aliquots to achieve desired final concentrations in a final volume of 1ml in each well. The fifth section of ovary was photographed on a Sedgewick rafter cell with a Jenoptik ProgRes® C5 microscope camera to characterize egg stages for each fish. Four plates containing n=4-5 replicates per treatment were incubated for 6 hours at 28 ℃, each plate completed on different days to replicate the assay temporally. After incubation, ovarian tissues were snap frozen in RNAlater™ (Invitrogen Cat# AM7020) for transcriptome analysis and culture media were stored in glass tubes at -80°C for further steroid quantification analysis via liquid chromatography tandem mass spectrometry (LC-MS/MS).","dates":{"publication":"2026/08/17"},"accession":"GSE299271","cross_references":{"GSM":["GSM9036476","GSM9036477","GSM9036478","GSM9036479","GSM9036483","GSM9036472","GSM9036484","GSM9036473","GSM9036485","GSM9036474","GSM9036475","GSM9036480","GSM9036481","GSM9036470","GSM9036482","GSM9036471"],"GPL":["30566"],"GSE":["299271"],"taxon":["Dichotomyctere nigroviridis"],"PMID":["[42563402]"]}}