{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE299nnn/GSE299448/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Mus musculus"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE299448"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"scRNA-Seq of CD45+ cells in B16F10 tumor after adoptive cytotoxic T lymphocyte immunotherapy","description":"Although adoptive cell transfer (ACT) with tumor-specific CD8⁺ T cells induces initial tumor regression, tumors often relapse due to incomplete eradication and immune evasion. To investigate the mechanisms of tumor regrowth after ACT, we performed single-cell RNA sequencing (scRNA-seq) of tumor-infiltrating immune cells in a murine melanoma model. B16F10 melanoma-bearing C57BL/6 mice were treated with gp100-specific Pmel-1 cytotoxic T lymphocytes (CTLs). Tumors were harvested on days 3, 7, and 14 post-transfer, and CD45⁺ immune cells were isolated for 10x Genomics Chromium-based scRNA-seq. This dataset supports the analysis of immune cell states during tumor progression after ACT.","dates":{"publication":"2026/04/26"},"accession":"GSE299448","cross_references":{"GSM":["GSM9039360","GSM9039359","GSM9039361"],"GPL":["28457"],"GSE":["299448"],"taxon":["Mus musculus"]}}