{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE299nnn/GSE299900/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Mus musculus"],"gds_type":[" Other","Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE299900"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Circulatory ILC2 convert and persist as part of the long-term tissue-resident pool after infection [Congenic Transfer]","description":"Circulating ILC2 can serve as a first line of defense against infection prior to the local expansion of lung-resident ILC2. The fate of these circulating ILC2 and their contribution to the long-term lung-resident ILC2 pool is not understood. Using reporter mice in combination with single cell RNA sequencing (scRNA-seq) and novel applications of Cellular Indexing of Transcriptomes and Epitopes sequencing (CITE-seq), we assessed vascular and parenchymal ILC2 populations during helminth infection and the fate of circulatory ILC2. These studies revealed that circulating ILC2 rapidly convert to a tissue-resident phenotype upon arrival in the lung. Once converted, former circulating ILC2 become largely indistinguishable from lung-resident ILC2 over time. Key gene expression retained by converted ILC2 at the peak of the tissue-resident ILC2 response was related to maintained IL-25-responsiveness and unique cytokine potential. These converted ILC2 accumulate, expand, and persist alongside conventional lung-resident populations even after repeated pulmonary infections. These findings reveal an important contribution of circulating ILC2 to the long-term health and maintenance of the lung-resident ILC2 population.","dates":{"publication":"2026/03/30"},"accession":"GSE299900","cross_references":{"GSM":["GSM9049519","GSM9049537","GSM9049526","GSM9049527","GSM9049538","GSM9049539","GSM9049528","GSM9049529","GSM9049518","GSM9049533","GSM9049522","GSM9049534","GSM9049523","GSM9049524","GSM9049535","GSM9049536","GSM9049525","GSM9049540","GSM9049541","GSM9049530","GSM9049531","GSM9049520","GSM9049521","GSM9049532"],"GPL":["30172"],"GSE":["299900"],"taxon":["Mus musculus"],"PMID":["[41847845]"]}}